在前期以EMS(甲基磺酸乙酯)筛选更多抗生物和非生物逆境的突变体试验中,发现了1个从G到A的点突变体rfc3-1,该点突变削弱了植株抗紫外辐射的能力.将野生型拟南芥复制因子C亚基3基因转化到突变株rfc3-1后恢复了突变株的野生型表型,证明紫外辐射抗性异常表型是由拟南芥复制因子C亚基3基因突变所引起的,AtRFC3在拟南芥紫外辐射抗性中起重要作用.
Replication factor C (RFC), consisting of one large subunit and four small subunits, is an important factor involved in DNA replication and repair mechanisms as well as cell proliferation. The subunit 3 of Arabidopsis RFC (AtRFC3) is one of the 4 small subunits. In a former EMS (ethylmethane sulfonate) screening to find out more mutant resistant to biotic and abiotic stresses, rfc3-1 was identified as a point mutant with G to A mutation occurred in the second exon which compromised the resistance of rfc3-1 plants to ultraviolet. Transformation of wild type AtRFC3 gene into rfc3-1 mutant reverted the mutant phenotypes, suggesting that AtRFC3 plays an important role in ultraviolet resistance in Arabidopsis thaliana.