中文摘要：

分别对离体新鲜的正常甲状腺组织、结节性甲状腺肿组织以及甲状腺乳头状癌组织进行自体双光子激发荧光(TPEF)和二次谐波(SHG)成像的研究.从双光子图像可知,正常甲状腺组织中滤泡大小较均匀,形态较相似,结节性甲状腺肿滤泡大小不等,而甲状腺癌组织则为大量的实质性癌细胞团结构,结果与标准组织学苏木精一伊红(H＆E)染色图相一致.此外,正常甲状腺组织和结节性甲状腺肿在胶原分布上存在一定差别.研究表明,双光子荧光成像技术可在微观结构上分辨出正常甲状腺组织、结节性甲状腺肿组织以及甲状腺乳头状癌组织的形态学差异,并有望成为甲状腺微创、快速诊断的有效方法.

英文摘要：

By using two-photon excited fluorescence (TPEF) and second harmonics generation (SHG), we imaged fresh ex vivo thyroid tissues, including normal thyroid tissue, nodular thyroid tissue and papillary thyroid carcinoma tissue. As can be seen, the follicles in normal thyroid tissue are in uniform size and shape, while in nodular thyroid tissue the follicles are in different size, and there are abundant substantial cancer cells in papillary thyroid carcinoma. These results are consistent with the corresponding standard H&E histology imagings. Furthermore, the collagen distributions in normal thyroid tissue and nodular thyroid tissue are observed to be different. These results suggest that the two-photon fluorescence microscopy imaging can distinguish, the morphologic differences between normal thyroid tissue, nodular thyroid tissue and papillary thyroid carcinoma tissue in microstructure, and it has the potential to be used for minimal invasive and rapid diagnosis of thyroid cancer.