中文摘要：

为研究延边黄牛体细胞核移植（SCNT）胚胎编码过氧化氢酶（CAT）、锰超氧化物酶（Mn-SOD）和谷胱甘肽过氧化物酶（GPx）的mRNA表达情况,以孤雌激活（PA）胚胎为对照,利用反转录PCR扩增延边黄牛SCNT胚胎编码CAT、Mn-SOD和GPx的mRNA,琼脂糖凝胶电泳后使用计算机软件进行半定量分析。结果显示：延边黄牛SCNT胚胎编码CAT的基因在2细胞期开始表达,且与PA胚胎存在显著差异（P〈0.05）;编码Mn-SOD的基因在8细胞期开始表达,与PA胚胎在16细胞期以上时期差异显著（P〈0.05）;编码GPx的基因在4细胞期开始表达,与PA胚胎在4细胞期差异显著（P〈0.05）,8细胞期以上时期差异不显著（P〈0.05）。与孤雌激活胚胎的差异表明SCNT胚胎抗氧化酶表达的异常可能是由核移植操作过程所引起的。

英文摘要：

To study the mRNA expression of encoding catalase（ CAT）,superoxide enzyme manganese（ Mn-SOD） and glutathione peroxidase（ GPx） of Yanbian cattle somatic cell nuclear transfer（ SCNT） embryos,the CAT,Mn-SOD and GPx encoding mRNA in SCNT embryos were amplificated by reverse transcription PCR.The parthenogenetic activation（ PA） embryos were used as the control group. After agarose gel electrophoresis,semi-quantitative analysis was carried out using computer softwares.The results showed that the gene encoding CAT in SCNT embryos began to express in the 2-cell stage,and it was significantly different（ P〈0.05） from PA embryos; the gene encoding Mn-SOD began to express in 8-cell stage,and significantly different from PA embryos above the 16-cell period（ P〈0.05）; the gene encoding GPx began to express at the 4-cell stage,there was significant difference compared with PA 4-cell stage embryos（ P〈0.05）,however,no more difference was found after the 8-cell stage（ P〈0.05）.These results indicate that the abnormal expression of antioxidant enzymes may be caused by nuclear transplantation operation during SCNT.