中文摘要：

目的：探讨游离脂肪酸（free fatty acid，FFA）对3T3-L1前脂肪细胞分化的影响及其可能的作用机制。方法：用FFA（100μg／ml）刺激小鼠成纤维细胞株3T3-L1；构建11β-羟化类固醇脱氢酶1（11β-hydroxysteroid dehydrogenase type 1．11β-HSD1）的SiRNA表达质粒pGCsilencer^TM H1／TetO1-11β—HSD1转染到3T3-L1细胞中并稳定表达，Western blot验证转染效率：采用荧光定量PCR检测脂肪细胞分化相关标志基因：过氧化物酶体增殖物激活受体γ（Peroxisome proliferator-activated receptorγ，PPARγ），CAATT增强子结合蛋白α（CAATT enhancer binding proteins α，C／EBPα）．脂蛋白脂酶（Lipoprotein lipase．LPL）和脂肪酸合成酶（Fatty acid synthetase，FAS）mRNA表达量的改变以及FFA刺激时11β-HSD1的基因表达水平。结果：FFA刺激后，分化指标PPARγ、C／EBPα、LPL和FAS的mRNA表达量增加，促进了脂肪细胞的分化（P〈0．01）；同时11β-HSD1基因的表达增加（P〈0．05）；11β-HSD1基因沉默后，分化指标的mRNA表达量降低，脂肪细胞的分化能力下降（P〈0．01）。结论：FFA和11β-HSD1都能促进脂肪细胞分化，FFA的成脂作用可能通过增加11β-HSD1的表达实现。

英文摘要：

Objective：To investigate the effects and mechanisms of free fatty acid （FFA） on 3T3-L1 preadipocyte differentiation. Methods： 1 β-HSD1-SiRNA expression plasmid （pGCsilencerTM H1/TetOl-1β-HSD1） was constructed and transfected into 3T3-L1 cells. 100 μg/ml FFA was added to the 3T3-L1 cells. Levels of adipogenesis-associated markers such as Peroxisome proliferatoractivated receptorγ （PPARγ）, CCAAT enhancer binding protein α （C/EBPα）, lipoprotein lipase （LPL）, and fatty acid synthetase （FAS） were measured. Results：When the cells treated with FFA, mRNA expressions of PPARγC/EBPα,LPL and FAS were upregulated and the expression of 11β-HSD1 also increased. When 11β-HSD1 was silenced, the levels of the markers were downregulated. Condusion：FFA and 11β-HSD1 could promote adipocyte differentiation, and the effect of FFA on adipogenesis carried out through increasing the expression of 1 β-HSD1.