中文摘要：

目的探讨中性粒细胞明胶酶相关脂质运载蛋白（NGAL）在肾间质纤维化（RIF）中的表达变化及其意义。方法60只SD雄性大鼠随机分为假手术（SOR）组、单侧输尿管梗阻（UUO）组和血管紧张素转换酶抑制剂（ACEI）组。EHSA法检测血清NGAL、肿瘤坏死因子（TNF）-α含量，免疫组化法检测肾组织NGAL、转化生长因子（TGF）-β1、基质金属蛋白酶-9（MMPO）的表达水平。结果（1）UUO组大鼠血清NGAL、TNF-α水平均显著高于ACEI组和SOR组[NGAL：（69．2±5．6）p∥ml比（41．0±10．4）pg／ml、（10．8±3．8）pg／ml，TNF-α：（116．2±9．2）ng／ml比（99．8±14．0）n∥ml、（29．2±5．7）ng／ml，均P〈0．05]，SOR组最低。（2）NGAL、TGF-β1、MMPO在UUO组大鼠肾小管间质内表达明显增强；在ACEI组，TGF-β1表达量低于UUO组，而NGAL、MMPO表达量在术后14d内均少于UUO组，但在术后21和28d其表达量高于UUO组。（3）相关关系：在UUO组，血清NGAL水平与TNF-α、血清肌酐呈显著正相关（r=0．910、0．673，P〈0．01）；肾脏NGAL表达量与MMPO表达水平呈显著正相关（r=0．913，P〈0．01），与肾小管间质损伤指数、TGF-β1表达阳性程度在术后3-7d呈显著正相关（r=0．937、0．847，P〈0．01），在术后14-28d呈显著负相关（r=-0．945、-0．944，P〈0．01）。结论NGAL在UUO大鼠血清与肾组织中的表达显著增加，并与MMP．9、TNF．d、TGF-β1等因子密切相关，而ACEI可通过多种机制影响NGAL在RIF中的生物学作用。

英文摘要：

Objective To explore the expression and significance of neutrophil gelatinase-associated lipocalin（NGAL） in renal interstitial fibrosis （RIF） in rats. Methods A total of 60 male Sprague-Dawley rats were randomly divided into 3 groups of sham operation （ SOR）, unilateral ureteral obstruction （UUO） and angiotensin-converting enzyme inhibitor （ACEI）. The serum concentrations of NGAL and tumor necrosis factor-alpha （TNF-α） were detected by enzyme-linked immunosorbent assay （ELISA）. And the expressions of NGAL, matrix metalloproteinase-9 （MMP-9） and transforming growth factor-β1 （TGF-β1） were observed by immunohistochemistry. Results （ 1 ） The levels of serum NGAL and TNF-α in UUO group obviously increased as compared to those in ACEI and SOR groups （ NGAL： （69. 2 _＋ 5.6 ） vs （41.0 _＋ 10. 4 ） , （ 10. 8 ± 3.8） pg/ml; TNF-ct： （ 116. 2 ± 9. 2） vs （ 99. 8 ＋ 14. 0）, （ 29. 2 ± 5.7 ） ng/ml; all P 〈 0. 05 ）. （ 2 ） The expressions of NGAL, TGF-β1 and MMP-9 in renal tubular epithelial cells of UUO group increased as compared to those in SOR group. The expression of TGF-β1 in ACEI group was apparently less than that in UUO group. The protein levels of NGAL and MMP-9 in ACEI group were obviously lower than those in UUO group within Day 14 post-operation and significantly higher than those in UUO group at Days 21 and 28. （3） In UUO group, the level of NGAL was positively correlated with the serum levels of TNF-α and serum creatinine（r =0. 910, 0. 673, P 〈 0. 01 ）. The expression of NGAL had a highly positive correlation withMMP-9（ r = 0. 913, P 〈 0.01 ）and the index of interstitial damage and the degrees of TGF-131 at Days 3 -7 post-operation（r = 0. 937, 0. 847, P 〈 0. 01 ）. And the expression of NGAL was negatively correlated with the index of interstitial damage and the degrees of TGF-β1 at Days 14 - 28 post-operation （ r = - 0. 945, -0. 944, P 〈 0. 01 ）. Conclusion The expression of NGAL significantly incre