中文摘要：

目的观察脾气虚大鼠骨骼肌组织Ca2+/钙调蛋白(Ca M)信号通路中关键分子〔Ca2+〕i以及Ca M、钙调蛋白激酶(Ca MK)Ⅱ、pCa MKⅡ蛋白表达水平的变化。方法受试动物随机分为正常对照组,脾虚模型7、14、21 d组,每组12只。除正常对照组外,其余受试动物采用复合法(苦寒破气法、游泳力竭法及饥饱失常法)成功建立脾气虚证大鼠模型,在观测各组大鼠一般生存状态、胃肠转运功能和骨骼肌组织ATP酶活性的基础上,采用激光共聚焦技术检测骨骼肌组织细胞内〔Ca2+〕i浓度,蛋白免疫印迹技术检测骨骼肌组织Ca M、Ca MKⅡ和p-Ca MKⅡ的表达变化。结果与空白组比较,脾气虚大鼠随着造模时间的延长,胃残留率升高而小肠推进率下降(P<0.01);骨骼肌组织Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性均降低(P<0.05,P<0.01);骨骼肌组织〔Ca2+〕i浓度和Ca M、Ca MKⅡ、p-Ca MKⅡ蛋白表达量显著降低(P<0.01);且脾虚模型7 d、14 d、21 d组之间比较,以脾虚21 d组变化更为显著。结论脾虚大鼠骨骼肌组织Ca2+/Ca M信号通路关键分子Ca M、Ca MKⅡ和p-Ca MKⅡ蛋白以低表达为主。

英文摘要：

Objective To investigate the expression changes of Ca 2+/CaM signaling pathways key factors〔Ca2+〕i,CaM,CaMKⅡand p-CaMKⅡin skeletal muscle tissue of rats with spleen-qi deficiency .Methods The rats were randomly divided into normal control ,spleen-qi deficient model groups(observed on 7 d,14 d and 21 d),12 rats in each.The spleen-qi deficient model rats were made by rhubarb,ex-haustive and hungry method ,then general existence ,gastric remnant rate ,intestinal propulsion rates ,and ATPase activity related to metabolism were evaluated.Also,confocal laser technology was used to test cellular 〔Ca2+〕i concentration and Western blot was used to test CaM ,CaMKⅡ,p-CaMKⅡexpressions in skeletal muscle of spleen asthenia rats .Results Compared with that of normal group ,gastric remnant rate was increased and small intestinal propulsion rates were decreased (P<0.01),the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase were de-creased significantly(P<0.01,P<0.05),and the expressions of CaM,CaMKⅡ,p-CaMKⅡin skeletal muscle tissue were decreased,these differences were obvious on spleen-qi deficient model 21 d group(P<0.01).Conclusions The 〔Ca2+〕i concentrations,CaM,CaMKⅡand p-CaMKⅡabnormaly decreased might be one of the pathological mechanisms of spleen -deficiency .