中文摘要：

以2年生银杏实生苗为试材，在人工气候室内采用土培盆栽试验方法，研究了温度和土壤水分对银杏叶黄酮类化合物积累的影响．试验设置土壤含水量（w）和温度（T）各3个梯度，w1、w2、W3分别为田间持水量的55％-60％、40％～45％、30％～35％；T1、T2、T3白天和夜间的温度分别为15／5℃、25／15℃、35／25℃．结果表明：T1温度条件下，各土壤水分处理的银杏叶中的槲皮素、山奈酚、异鼠李素和总黄酮含量普遍高于T2和T3，而土壤水分对银杏叶中各种黄酮类化合物积累的影响不显著；银杏叶中黄酮类化合物以山奈酚含量最高，其次为槲皮素和异鼠李素；T3温度下银杏单株总黄酮产量普遍高于T2和T3．在收获前适当采取土壤覆盖和灌水等措施降低种植园的温度，有利于提高银杏叶中黄酮的含量，增加单位面积黄酮的产量．

英文摘要：

Taking the 2-year old Ginkgo biloba seedlings as test materials, a pot experiment was conducted in an artificial climate chamber to study the effects of air temperature and soil moisture on the flavonoids accumulation in leaves. Three levels of air temperature （ 15/5 ℃, 25/15 ℃, and 35/25 ℃ day/night） and three levels of soil moisture （55%-60% , 40%-45% , and 30%-35% of field capacity） were installed, yielding nine temperature-soil moisture combinations. Under the three levels of soil moisture, the quercetin, kaempferol, isorhamnetin, and total flavonoids contents in the leaves were higher at 15/5 ℃ than at 25/15 ℃ and 35/25 ℃. Soil moisture had minor effects on the flavonoids accumulation. The leaf kaempferol content was the highest, followed by quercetin and isorhamnetin. The total flavonoids yield per plant at 35/25 ℃ was higher than that at 15/5 ℃ and 25/15 ℃. It was suggested that to adopt appropriate soil covering and watering before harvesting to decrease the ambient temperature could benefit the enhancement of leaf flavonoids con- tent and the improvement of per unit area flavonoids production in G. biloba leaf-harvesting planta- tion.