本研究通过酵母双杂交技术,构建了IrrE蛋白为诱饵载体,从拟南芥cDNA文库中筛选与IrrE诱饵蛋白相互作用的功能蛋白.研究结果表明,在所获得的阳性克隆中,发现了一个与拟南芥基因Atlg01470所编码的蛋白有较高同源性的蛋白,即LEAl4蛋白(晚期胚胎发育富集蛋白),同源性高达98%.推测IrrE转录因子在提高植物耐盐性的过程中起了重要作用.
. IrrE was constructed as the bait protein and then interacted with functional proteins from the Arabidopsis cDNA library by yeast two-hybrid system. The result showed that there was one interacting protein from all the positive clones which had high identity (as high identical to 98%) with the LEA14 protein (Late Embryogenesis Abundant protein 14) encoded by Arabidopsis thaliana gene Atlg01470. It was inferred that IrrE protein may play an important role in improving the tolerance to salt stress in trans-gene plants.