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中文摘要：
目的通过研究E3泛素连接酶FBXL20及其下游蛋白-神经元突触膜胞外分泌调节蛋白1（regulating synaptic membrane exocytosis 1,RIM1）在颞叶癫痫患者皮质及匹鲁卡品癫痫大鼠模型皮质、海马组织中的表达变化,探讨FBXL20在癫痫形成中的作用。方法以颞叶癫痫患者手术切除病灶标本（n=25）为癫痫组,以脑外伤减压手术所切皮质标本（n=10）为对照组。另取40只健康雄性SD大鼠腹腔注射氯化锂（127 mg/kg）,20 h后腹腔注射匹鲁卡品（50 mg/kg）,每30分钟重复给予1次匹鲁卡品（10 mg/kg）,直到大鼠癫痫发作。2周后出现癫痫自发性发作的大鼠纳入癫痫组;2个月后仍未出现癫痫自发性发作的大鼠纳入对照组。采用免疫荧光对FBXL20的表达进行定位;免疫组化检测FBXL20的表达水平;Western blot检测FBXL20、RIM1的表达水平。结果免疫荧光检测结果显示FBXL20主要表达于神经元;免疫组化及Western blot检测结果均显示癫痫患者皮质、癫痫大鼠模型皮质与海马中FBXL20表达较对照组明显下降（P〈0.05）;同时Western blot检测结果显示RIM1表达较对照组明显升高（P〈0.05）。结论 FBXL20在癫痫患者及大鼠模型中的表达水平明显降低,RIM1表达明显升高,提示FBXL20可能通过调控RIM1参与了癫痫的形成。
英文摘要：
Objective To investigate the role of E3 ubiquitin ligase FBXL20 in the epileptogenesis through detecting the expression level of FBXL20 and its downstream regulating synaptic membrane exocytosis1（ RIM1） in brain tissue of temporal lobe epilepsy patients and pilocarpine-induced rat model. Methods Epilepsy focus resected from temporal lobe epilepsy patients was taken as the epilepsy group（ n = 25）,and the cortex resected from brain trauma patients was taken as the control group（ n = 10）. Forty male,healthy SD rats were injected with pilocarpine（ 127 mg / kg）. The rats with spontaneous seizures in 2 weeks later were selected into the epilepsy model group,while those without spontaneous seizures until 2 months later were selected into the control group. FBXL20 and RIM1 in the human brain cortex and rat cortex and hippocampus were detected by immunofluorescence staining,Western blotting and immunohistochemistry assay. Results Immunofluorescence staining showed that FBXL20 mainly located in the neurons. Immunohistochemistry assay and Western blotting showed that the expression of FBXL20 was down-regulated in the epilepsy group（ P 〈0. 05）. Western blotting indicated that expression of RIM1 was up-regulated in the epilepsy group（ P〈 0. 05）.Conclusion Altered expression of FBXL20 indicates that FBXL20 may be involved in the epileptogenesis.
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