中文摘要：

目的探讨芥菜籽提取物抗氧化、调节CD36的表达及预防动脉粥样硬化的作用。方法将C57／BL6小鼠随机分为对照组和芥菜籽组，分别经腹腔给予1．5％的生理盐水、1．5％芥菜籽提取物溶液，连续3天，第4天停止注射，第5天经眼眶取血并处死老鼠，取腹腔巨噬细胞培养。将培养的巨噬细胞给予氧化型低密度脂蛋白刺激，观察腹腔巨噬细胞氧化型低密度脂蛋白诱导的巨噬细胞呼吸爆发和脂质过氧化物含量，清道夫受体CD36表达水平以及中性脂肪蓄积情况，并观察血浆超氧化物歧化酶活性和脂质过氧化物含量。雄性载脂蛋白E-/-小鼠30例．随机分为对照组、高脂组和高脂＋7．5％芥菜籽组进行饲养，25周后处死，分离并观察主动脉脂质斑块面积。结果芥菜籽提取物明显增加机体血浆超氧化物歧化酶水平和降低脂质过氧化物水平；降低氧化型低密度脂蛋白诱导的呼吸爆发；降低氧化型低密度脂蛋白诱导的脂质过氧化物升高及CD36的表达水平，减少细胞中性脂肪蓄积泡沫细胞形成；降低高脂引起的脂质斑块面积。结论芥菜籽提取物可有效地提高机体的抗氧化能力，提高巨噬细胞的抗氧化性，减少脂质的摄取，预防动脉粥样硬化早期病变。

英文摘要：

Aim To investigate the function of Sinapis alba extract＇ s antioxidant ability and regulation of CD36 expression in macrophages, and Sinapis alba extract＇ s prevention of atherosclerosis. Methods C57/BL6 mice were divided into two groups which were injected with 1.5% normal saline or 1.5% Sinapis alba extract for 0. 2 mL/day, respectively. The injection was paused for one day after continuously injected for three days, and then on the 5th day, mice were sacrificed to collect abdominal macrophages. Plasma superoxide dismutase （SOD） activity and lipid peroxide （LPO） ; cellular SOD, respiratory burst, LPO, expression of CD36 and neutral fat accumulation were assayed. 30 male ApoE -/- mouse were randomly divided into three groups： chew food diet group, high-fat diet group and high-fat diet ＋ 7.5% Sinapis alba group. The mouse were sacrificed 25 weeks later, and aorta was dissected, stained by oil red O. Plaque area was measured by an automatic image analyzer. Results In Sinapis alba extract group, SOD activity was higher and LPO was lower than that in the control group. Sinapis alba extract could reduce macrophage respiratory burst, and LPO level, CD36 expression and neutral fat accumulation induced by ox-LDL. Sinapis alba extract could reduce lipid plaque area by high-fat diet （ P 〈 0. 05 ）. Conclusions Sinapis alba extract can effectively improve the body＇ s antioxidant capacity, improve the antioxidant activity of macrophages, reduce lipid uptake, prevent early atherosclerosis lesions.