植物线虫可危害农作物和林木,对它们的准确鉴定是防治植物线虫病害的基础。由于植物线虫很小,而且在形态上种间常有覆盖,而种内有较大的变异,仅依据形态特征很难鉴定。分子鉴定技术给植物线虫的检测和鉴定提供了快速、精确、可靠的方法。文章综述了植物线虫分子的 DNA 提取、分子鉴定靶标序列的选择及分子鉴定方法等方面的研究进展及现状,以促进对植物线虫分子鉴定更深入的研究。
Plant nematodes can damage crops and trees.The accurate identification of plant nematodes is the basis for the control of plant nematode diseases.It is very challenging to identify plant nematode species only based on morphology,because plant nematodes are microscopic in size,and some morphological characters may have inter-specific overlapping or intraspecific variability.Molecular techniques provide a fast,accurate and reliable method for the detection and identification of plant nematodes.For promoting the deep study on molecular identification of plant nematodes,this paper summarized research progresses in and current research status on DNA extraction of plant nematodes,target sequences for identifying plant nematodes and the main molecular identification meth-ods for plant nematodes.