中文摘要：

采用PCR和DNA测序技术对贵州都柳江鲤（Cyprinus carpio）、鲫（Carassius auratus）和草鱼（Ctenopharyngodon idellus）种群的mt DNA控制区序列及遗传多样性进行了研究。获得了都柳江鲤、鲫和草鱼mt DNA控制区长度分别为899~901 bp、787 bp和901~905 bp的序列。该3种鱼类控制区碱基A、T含量较高,G含量最低。识别了该3种鱼类mt DNA控制区终止序列区、中央保守区和保守序列区等保守序列。其中,除CSB-2和CSB-3碱基组成相同外,其余核心序列碱基组成存在着差异。都柳江鲤、鲫和草鱼种群mt DNA控制区分别有24、24和11个多态位点,分属12、17和8个单倍型。都柳江鲤、鲫种群遗传多样性较高,草鱼种群遗传多样性较低。因此,有必要开展都柳江草鱼种群遗传多样性的保护。

英文摘要：

The mtDNA D-loop and genetic diversity of the populations of Cyprinus carpio, Carassius auratus and Ctenopharyngodon idellus in Duliu River, Guizhou were studied by the methods of PCR and DNA sequencing in this research. We found that the mtDNA D-loop length of Cyprinus carpio, Carassius auratus and Ctenopharyngodon idellus were 899-901 bp, 787 bp and 901-905 bp, respectively. The content of bases A and T was high in the mtDNA D-loop of the 3 populations, but that of G was the lowest. Also, the extended termination associated sequence （ETAS）, the central conserved domain （CD） and the conserved sequence block （CSB） in the mtDNA D-loop of the 3 populations were diagnosed. Moreover, except for the same base composition of CSB-2 and CSB-3, there were differences in the nucleotide composition of the other core sequences. There were 24, 24 and 11 polymorphic sites in the mtDNA D-loop of Cyprinus carpio, Carassius auratus and Ctenopharyngodon idellus, and they belonged to 12, 17 and 8 haplotypes, respectively. The populations of Cyprinus carpio and Carassius auratus had high genetic diversity, whereas the population of Ctenopharyngodon idellus had much lower genetic diversity. Therefore, it was necessary to protect the population of Ctenopharyngodon idellus in Duliu river.