中文摘要：

采用改进的RNA指纹技术（RAP—PCR）对两套大白菜胞质雄性不育材料及其杂种F1花蕾的总RNA进行了分析，通过对186条随机引物的筛选，获得4个重复性较好的差异片段S47—412，S93—622，S176—343，S199—904，并对其克隆、测序。根据各差异条带的测序结果合成长引物进行验证，S47，S93所对应的差异消失，而S176，S199长引物验证的结果与RAP—PCR相似，序列分析表明：S176—343，S199—904两差异片段均和油菜Polima不育胞质线粒体基因组orf224／atp6位点有着极高的同源性，两差异片段序列部分重叠，这说明两差异片段可能和大白菜胞质雄性不育有很高的相关性。

英文摘要：

In order to investigate the differential expression of the genes related to cytoplasmic male sterility in Chinese cabbage （Brassica campestris L. ssp. Penkinsis）, a modified RNA fingerprinting technique was developed to compare the difference in the total RNA from flower bud of Chinese cabbage among cytoplasmic male sterility （CMS） lines, maintainer lines and F1 hybrids. Four stably differential fragments S47-412, S93-622, S176-343 and S199-904 were amplified, cloned and sequenced with primers selected from 186 random primers. Based on the nucleotide sequence of the four differential fragments, four pairs of specific primers were designed to validate the differential fragments. The validation showed S47-412 and S93-622 were false positives and S176-343 and S199-904 were confirmed by PCR with the specific primers. Sequence analysis revealed that both of two differential fragments had strong homology with the nucleotide sequence of orf224/atp6 site of Polima CMS and the nucleotide sequence of S176-343 and S199-904 had a superposed region. All these indicate that the two fragments probably have strong relationship with cytoplasmic male sterility in Chinese cabbage.