位置：成果数据库 > 期刊 > 期刊详情页
人的脐的间充质的干细胞和它的 adipogenic 区别: 由原子磁性的回声光谱学介绍
  • ISSN号：1008-1062
  • 期刊名称：《中国临床医学影像杂志》
  • 时间：0
  • 分类：R329[医药卫生—人体解剖和组织胚胎学;医药卫生—基础医学]
  • 作者机构：[1]Zhi-Feng Xu, Yao-Wen Chen, Ren-Hua Wu, Department of Medical Imaging, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, Guangdong Province, China, [2]Zhi-Feng Xu, Ai-Zhen Pan, Fang Yong, Department of Medical Imaging, The First People’, s Hospital of Foshan, Foshan 528000, Guangdong Province, China, [3]Chong-Yang Shen, Multidisciplinary Research Center of Shantou University, Shantou 515041, Guangdong Province, China
  • 相关基金：Supported by Grants from the National Natural Science Foundation of China(Key program 30930027);Natural Science Foundation of Guangdong Province(No.8151503102000032)
作者： Zhi-Feng Xu, Ai-Zhen Pan, Fang Yong, Chong-Yang Shen, Yao-Wen Chen, Ren-Hua Wu
关键词： Human, UMBILICAL, MESENCHYMAL, stem, cell, Adipogenic, DIFFERENTIATION, MR, SPECTROSCOPY, BIOMARKER, Human umbilical mesenchymal stem cell, Adipogenic differentiation, MR spectroscopy, Biomarker
中文摘要：

AIM:To study the metabolic profile of human umbilical mesenchymal stem cells(HUMSC) and adipogenic differentiation by nuclear magnetic resonance(NMR) spectroscopy.METHODS:HUMSC isolated from human umbilical cord stroma were induced to adipocytes over 2 wk by adding dexamethasone,3-isobutyl-1-methylxanthine,indomethacin,and insulin to the culture medium.Adipogenic differentiation was confirmed by Red O staining and transcription-polymerase chain reaction.Perchloric acid extracts of the HUMSCs and adipocytes(about 7 ×10 6) were characterized for metabolites by using in vitro high resolution 9.4T NMR spectroscopy.RESULTS:Several major metabolites,such as:choline,creatine,glutamate and myo-inositol,acetate,and some fatty acids/triglycerides,were observed in the MR spectroscopic pattern of HUMSCs and their adipogenic differentiation.HUMSCs are characterized by an unusually low number of NMR-detectable metabolites,high choline,acetate,glutamate and creatine content.However,the metabolic profiles of adipogenic differentiation demonstrated considerably higher methionine and fatty acids,and non-detectable creatine.CONCLUSION:The biomarkers of HUMSCS and adipocytes were obtained and assigned.NMR spectroscopy will be a promising tool for monitoring stem cell differentiation.

英文摘要：

AIM: To study the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and adipogenic differentiation by nuclear magnetic resonance (NMR) spectroscopy.

同期刊论文项目
准确无损地测定脑代谢物浓度和pH值的1H和31P磁共振频谱成像研究
期刊论文 71 会议论文 51
同项目期刊论文
7.0T功能MR波谱动态定量检测视觉皮层代谢物对视觉刺激的反应
Improved measurement of labile proton concentration-weighted chemical exchange rate (kws) with exper
Quantitative multivoxel proton MR spectroscopy study of brain metabolites in patients with amnestic
Evaluation of the dependence of CEST-EPI measurement on repetition time, RF irradiation duty cycle a
Quantification of Choline Concentration Following Liver Cell Apoptosis using1 H Magnetic Resonance S
Human umbilical mesenchymal stem cell and its adipogenic differentiation profiling by nuclear magnet
基于pH值敏感的磁化传递技术在1．5T磁共振成像上的研究
二维多体素波谱定量分析遗忘型轻度认知功能障碍患者颅内代谢物
Simultaneous determination of labile proton fraction ratio and exchange rate with irradiation radio
Advanced nasopharyngeal carcinoma radiotherapy with volumetric modulated arcs and the potential role
代谢综合征患者的脑MR波谱研究
代谢综合征患者的MR扩散张量成像研究
非痴呆型血管性认知功能损害和轻度认知功能损害患者颅内代谢物的定量研究
基于 9.4 T1H—MRS 和模式识别的大肠癌粪便代谢组学的研究
压缩感知技术及其在 MRI 上的应用
7.0 T MRI 体外标记 Gd-DTPA 对骨髓间充质干细胞的实验研究
Quantitative Proton Magnetic Resonance Spectroscopy of Human Precentral Gyrus and Hippocampus: Absol
MRS双量子相干滤波技术在体检测GABA和GSH的研究现状
电针刺激脊髓损伤患者穴位的脊髓磁共振功能成像激活区特征
磁共振pH成像的研究
医学影像学教学中激励医学生自主学习的探索
Electrophysiological correlates of proactive interference in the 'Recent Probes' verbal working memo
磁共振频谱检测GABA的模拟和临床模型研究
STEAM和PRESS序列对磁共振频谱绝对定量的影响
稳定期双相情感障碍病人经丙戊酸钠治疗脑肌醇无显著意义的变化:在体磁共振频谱分析(英文)
应用~1H-MRS技术结合LCmodel软件定量分析正常成人中央前回及海马脑代谢物浓度
磁共振频谱数据定量后处理技术比较
应用64层螺旋CT定量局部左室功能正常参考值
用于高场 MRS 研究的人脐带间充质干细胞代谢物提取方法选择的优化
平山病颈椎 MRI 影像学分析
7．0 T功能MR波谱动态定量检测视觉皮层代谢物对视觉刺激的反应
化学交换饱和转移在磁共振pH成像方面的应用
Helical CT findings and clinicopathologic features in malignant gastrointestinal stromal tumors: the
9.4T MRS观察人脐带间充质干细胞代谢特征
孤独症的神经系统研究进展
正常人海马短回波~1H-MRS0.5～2.05ppm区域特征性研究
MRS定量测量急性缺氧缺血脑损伤猪脑代谢物研究
MR Spectroscopy Study of Amnestic Mild Cognitive Impairment and Vascular Cognitive Impairment with N
Transarterial embolization using pingyangmycin lipiodol emulsion and polyvinyl alcohol for the treat
The 1.28 ppm biomarker: not specific for neural progenitor cells, but also in the mesenchymal stem c
Anterior cingulate cortex and cerebellar hemisphere neurometabolite changes in depression treatment:
MR Spectroscopy in Metabolic Profiling Studies of Stem Cells
Extraction methods-based analysis of the human umbilical cord mesenchymal stem cells on 9.4 T high-r
1H-Proton Magnetic Resonance Spectroscopy Differentiates Dementia with Lewy Bodies from Alzheimer&rs
Chemical Exchange Saturation Transfer (CEST) Imaging of pH
MEGA-PＲESS 序列定量检测在体人脑γ － 氨基丁酸浓度
电针刺激穴位的脊髓fMRI初步研究
Quantitative chemical exchange saturation transfer (qCEST) MRI - RF spillover effect-corrected omega
铜代谢紊乱对脑神经系统疾病的影响
Upregulation of adenosine A2A receptors induced by atypical antipsychotics and its correlation with
Image-guided percutaneous lipiodol-pingyangmycin suspension injection therapy for sacral chordoma
基于体素的形态测量学观察两种亚型血管性认知障碍患者脑灰质萎缩的差异
基于AMARES和QUEST的31P MRS绝对定量及比较.中国医学影像技术
Hippocampal Neurochemical Changes in Senescent Mice Induced with Chronic Injection of D-Galactose an
Behavioral deficits, abnormal corticosterone, and reduced prefrontal metabolites of adolescent rats
9.4T~1 H-MRS定量分析三种乳腺癌细胞的水溶性代谢物
9.4T MRS观察阿尔茨海默病模型小鼠海马代谢物早期变化.
抗精神病药对精神分裂症患者血小板腺苷A2a受体基因表达的影响
9.4T MR波谱观察离体间充质干细胞死亡的代谢特征
磁共振频谱技术及其在alzheimer病及极早期阶段检测颅内代谢物的应用
9.4T MRS观察阿尔茨海默病模型小鼠海马代谢物早期变化
应用1H-MRS技术结合LCmodel软件定量分析正常成人中央前回及海马脑代谢物浓度
化学交换饱和传递磁共振成像建模与仿真
基于7．0T磁共振用MT成像新方法探究NOE及APT加权成像
重视功能与分子医学影像学的临床应用前景研究
眶尖区外伤CT和MRI的诊断及比较
基于压缩感知的主成分分析7．0T磁共振稀疏图像快速重构
基于磁共振技术的脑肿瘤pH检测方法的现状及临床应用前景
核磁共振频谱对探测凋亡细胞代谢物变化的研究
期刊信息
  • 《中国临床医学影像杂志》
  • 北大核心期刊（2014版）
  • 主管单位:中华人民共和国卫生部
  • 主办单位:中国医科大学 中国医学影像技术研究会
  • 主编：郭启勇
  • 地址：沈阳市和平区三好街36号
  • 邮编：110004
  • 邮箱：jccmisy@sina.cn
  • 电话：024-23925069
  • 国际标准刊号：ISSN：1008-1062
  • 国内统一刊号：ISSN：21-1381/R
  • 邮发代号:8-25
  • 获奖情况:
  • 国家“双百”期刊,1998~2001年省科技期刊一等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2014版）
  • 被引量:17604