中文摘要：

Ebola 病毒(EBOV ) 和 Marburg 病毒(MARV ) 是有在人和非人类的首领的高死亡率的严重出血性的发烧的原因的代理人，当前没有准许的疫苗或治疗学。迄今为止，没有特定的实验室中国的诊断测试，当有一个公民时，需要在爆发期间并且为开创可接受的检疫过程提供微分诊断。在这研究，指向扎伊尔 Ebolavirus (ZEBOV ) 和 MARV 的核蛋白基因的 TaqMan RT-PCR 试金被开发，他们的敏感和特性被调查。我们的结果显示试金能用 103 ～ 109 在大量病毒拷贝上做可靠诊断，相应于一个标准 RNA 抄本的阀值。结果证明每病毒文化上层清液的毫升有大约 1010 个 RNA 拷贝，等价于 10,000 个 RNA 分子每传染 virion，建议许多非传染的粒子的存在。这些数据显示在这研究开发的 TaqMan RT-PCR 试金将在中国对未来监视和 ZEBOV 和 MARV 的特定的诊断合适。

英文摘要：

Ebola virus （EBOV） and Marburg virus （MARV） are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus （ZEBOV） and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 103 to 109, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 101 RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable