中文摘要：

鸡毒支原体（MG）是造成鸡慢性呼吸道疾病的主要病原菌。通常采用血清学方法诊断该病，由于存在非特异性交又反应，这给临床诊断带来困难。为解决MG快速诊断问题，该研究根据已发表的鸡毒支原体种特异性序列FMG-2合成一对引物（MG1，MG2），用PCR方法检测MG。结果表明，该PCR方法对MG能特异性扩增732bp目的片断，而对照菌（大肠杆菌、金黄色葡萄球菌等）却不能扩增出目的片段。PCR产物经测序，其序列与Genbank序列同源性为98．86％。用PCR方法检测60份感染样本，其阳性检出率为80％，而用传统的分离培养方法检出率仅为50％。

英文摘要：

Mycoplasma gallisepticum（MG） is the etiologic agent of avian chronic respiratory disease. Traditional serologic test for the diagnosis of the disease has encountered with cross-reaction. Therefore, it is difficult to diagnose MG infection with serologic methods. In order to solve this problem ,a PCR method based the reported DNA sequence of FMG-2 to detect MG was developed. A 732-bp expected fragment was amplified from MG DNA, but it was not amplified from other bacteria （including E.coli,S.aureus,MS,et al ）. Products of PCR from a reference strain and an isolate were sequenced .In comparison with the sequences in GenBank, the homology was 98.86%.Among the 60 clinical samples, the positive ratio was 80% for PCR, but the positive ratio was only 50% for isolation method.