中文摘要：

目的：研究携带绿色荧光蛋白（Green Fluorescent Protein，GFP）基因的重组腺病毒在大鼠骨髓间充质干细胞（MSCs）中的表达。方法：将纯化的GFP重组腺病毒分别用单次转染和多次转染两种不同方法转染MSC，观察其感染效率和GFP表达水平。结果：培养的MSC呈CD90和CD105强阳性，且具有成骨、成脂肪等多向分化能力；携带绿色荧光蛋白基因的重组腺病毒单次转染和多次转染P3代MSC都呈现出浓度依赖性；单次转染在80MOI时转染效率最高，而多次转染在20MOI时转染效率即已达90％。结论：建立稳定、高效表达Ad．GFP的骨髓间充质干细胞实验体系，为MSC的标记及示踪奠定基础。

英文摘要：

Objective To investigate the expression of recombinant adenovirus encoding green fluorescent protein（GFP） in mesenchymal stem cells （MSCs）. Methods The purified recombinant adenovirus encoding GFP （Ad-GFP） was transfected into MSCs with single or multiple infection, the transfer efficiency of Ad-GFP and the expression level of GFP were measured. Results Cultured MSCs were observed with a multiple differentiation potential into osteoblast ,lipoblast and highly expressed CD105 and CDgO. The Ad-GFP transfeetion of P3 generation MSCs with single or multiple ways were all shown in a concentration-dependent manner. The efficiency of MSCs transfected with Ad-GFP were about 90% in 80 MOI by single infection and in 20 MOI by muhiple infection. Conclusion The system of Ad-GFP expression in MSCs was established successfully and it could provide a basis for further resarch on MSCs labelling and tracing in transplantation experiment.