中文摘要：

目的 通过测定新疆维吾尔族艾滋病相关型卡波西肉瘤（AIDS-KS）和经典型KS患者肿瘤组织中Caspase-3、E-cad蛋白，进一步明确不同类型KS的发病机制。 方法 2011年7月至2014年10月新疆医科大学第一附属医院及乌鲁木齐市传染病医院就诊的KS患者的活组织检查标本38例，其中男28例，女10例，均为维吾尔族。38例患者中AIDS-KS 22例，经典型KS 16例。采用免疫组织化学法和蛋白质印迹法检测两组患者KS组织中Caspase-3、E-cad蛋白的表达。正态分布的计量资料采用t检验，计数资料两两比较用行×列表χ2检验。 结果 经典型KS患者KS病变仅限于皮肤，无黏膜、淋巴结或内脏受累表现。AIDS-KS患者病变不仅局限于皮肤及浅表淋巴结，12例口腔黏膜受累，7例受累内脏器官，以肝、肺受累多见。AIDS-KS患者CD4＋T淋巴细胞计数为（200.8±166.0）/μL，其中〈200/μL者15例，且均发生机会性感染；经典型KS患者CD4＋T淋巴细胞计数为（562.52±222.66） /μL，〉350/μL者16例，无患者发生机会性感染。采用免疫组织化学法检测显示，AIDS-KS患者KS组织中Caspase-3蛋白表达阳性率为68.2%，经典型KS患者表达率为100.0%，两组比较差异有统计学意义（χ2＝7.37，P＝0.01）；AIDS-KS患者KS组织中E-cad蛋白表达阳性率为72.7%，经典型KS患者表达率为100.0%，两组比较差异有统计学意义（χ2＝5.18，P＝0.03）。蛋白质印迹法检测显示，AIDS-KS患者KS组织中Caspase-3灰度值为0.55±0.36，经典型KS患者为0.86±0.56，两组比较差异有统计学意义（t＝－2.070，P〈0.05）；AIDS-KS患者KS组织中E-cad灰度值为0.54±0.41，经典型KS患者为0.85±0.45，两组比较差异有统计学意义（t＝－2.060，P〈0.05）。 结论 Caspase-3、E-cad在新疆维吾尔族AIDS-KS与经典型KS患者KS组织中的表达存在差异，可能与AIDS-KS具有更快的进展速度和更高的病死率相关。

英文摘要：

Objective To further clarify the pathogenesis of different types of Kaposi′s sarcoma （KS） by measuring the protein expressions of caspase-3 and E-cad in tumor tissues of Xinjiang Uygur patients with acqured immunodeficiency syndrome （AIDS）-Kaposi′s sarcoma （KS） and classical KS.Methods From July 2011 to October 2014, 38 patients with KS at the First Affiliated Hospital of Xinjiang Medical University and Urumqi Infectious Disease Hospital were enrolled, among whom 28 were male and 10 were female, and all of them were uygur. Immunohistochemical and Western blot methods were used to detect the expressions of caspase-3 and E-cad proteins in 22 cases of AIDS-KS patients and 16 cases of classic KS. The quantitative data of normal distribution were analyzed by t test, while count data were compared with χ2 test with R × C table.Results KS lesions in patients with classic KS were confined to the skin, without mucosal, lymph node or visceral involvement. Lesions in AIDS-KS patients were not only confined to the skin and superficial lymph nodes, but also oral mucosa involved in 12 cases and internal organs involved in 7 cases. Liver and lung involvement was more common. The CD4＋ T lymphocyte count in patients with AIDS-KS was （200.8±166）/μL. All 15 AIDS cases with CD4＋ T cell count less than 200/μL developed opportunistic infections.CD4＋ T lymphocyte count of patients with classic KS was （562.52±222.66）/μL and the 16 patients with CD4＋ T lymphocyte count greater than 350/μL had no opportunistic infections.The results of immunohistochemistry showed that the positive expression rate of caspase-3 protein in KS tissues in patients with AIDS-KS was 68.2%, in patients with classic KS was 100.0%, with significant difference between two groups （χ2=7.37, P=0.01）. The positive expression rate of E-cad protein in KS tissues in patients with AIDS-KS was 72.7%, in patients with classic KS was 100.0%, with significant difference between two groups （χ2=5.18, P=0.03）. Western blotting show