中文摘要：

目的探讨胰腺应激蛋白胰石蛋白/再生蛋白（PSP/reg）对胰腺星状细胞（PSC）的增殖、迁移、凋亡、胞外基质（ECM）合成的影响。方法分离纯化慢性胰腺炎患者纤维化区的PSC,基因重组胰腺应激蛋白PSP/reg,分别以10、100 ng/ml的终浓度对其进行干预。BrdU标记法检测PSC增殖;创伤愈合法观察PSC的迁移;原位缺口末端标记（TUNEL）技术检测PSC凋亡。细胞免疫荧光法观察细胞外基质（ECM）如胶原蛋白I（Col I）、纤连蛋白（FN）的生成。结果胰腺应激蛋白PSP/reg呈浓度依赖性地抑制PSC的增殖和迁移（P〈0.05）,而不影响PSC凋亡。免疫荧光结果显示Col I、FN生成减少。结论 胰腺应激蛋白PSP/reg在急慢性胰腺炎时显著升高。其抑制纤维化主要效应细胞PSC的活性,可能减少胰腺纤维化发生,在胰腺炎后的再生修复过程中发挥重要作用。

英文摘要：

Objective To observe the effects of pancreatic stress protein（PSP/reg） on the proliferation,migration,apoptosis and extracellular matrix（ECM） synthesis of pancreatic stellate cell（PSC）.Methods PSC was isolated and purified from the fibrotic human pancreas tissues.PSP/reg expressed in the yeast Pichia pastoris.PSP/reg was added at concentrations of 10 and 100 ng/ml to cultured PSC.Cell proliferation was determined by bromodeoxyuridine incorporation.PSC migration was assessed by wound healing assay.Cell apoptosis was shown by TUNEL assay.ECM（collagen and fibronectin） synthesis was examined by immunofluorescence staining.Results PSP/reg inhibited proliferation and migration of PSC in a dose-dependent manner（P0.05）,but did not induce apoptosis.PSP/reg reduced collagen and fibronectin synthesis.Conclusion PSP/reg is upregulated significantly during acute and chronic pancreatitis.By inhibiting PSC activity,PSP/reg may reduce fibrogenesis and have a protective function in the repair phase of pancreatic injury.