中文摘要：

目的：研究Runx1在骨形态发生蛋白9（bone morphogenetic proteins 9,BMP9）诱导小鼠胚胎成纤维细胞（mouse embryonic fibroblasts,MEFs）成骨分化中的作用。方法：用BMP9腺病毒感染MEFs细胞,利用RT-PCR和Western blot分别在mRNA和蛋白质水平检测Runx1的内源性表达;构建过表达Runx1的重组腺病毒Ad-Runx1,并在mRNA和蛋白质水平验证Ad-Runx1的效果;用Ad-Runx1和BMP9条件培养基共处理MEFs,检测成骨早期指标碱性磷酸酶（ALP）染色和活性,茜素红S染色检测成骨晚期指标钙盐沉积;RT-PCR和Western blot分别检测成骨关键转录因子Runx2在mRNA和蛋白质水平的变化。结果：Ad-BMP9处理MEFs细胞后,可使Runx1在mRNA和蛋白质水平表达上调;构建的Ad-Runx1处理MEFs后,可使Runx1在mRNA和蛋白质水平表达上调;Ad-Runx1处理BMP9诱导的MEFs细胞后,增强了ALP活性和钙盐沉积以及Runx2在mRNA和蛋白质水平的表达。结论：Runx1可以促进BMP9诱导的间充质干细胞MEFs的成骨分化。

英文摘要：

Objective： To investigate the role of Runx1 gene on BMP9-incuced osteogenic differentiation of Mouse embryonic fibroblasts（ MEFs）. Methods： MEFs were infected with Ad-BMP9 and then the expression of endogenous Runx1 at the mRNA and protein level was determined by RT-PCR and Western blot. The recombinant adenovirus Ad-Runx1 was constructed and its expression was validated at the mRNA and protein level. MEFs cells were treated with Ad-Runx1 or/and BMP9,the ALP activity was detected by quantitative and staining assay,calcium deposition was detected by Alizarin Red S staining. Runx2,as a core osteogenic transcription factor,was detected by RT-PCR and Western blot at the mRNA and protein level. Result： The expression of Runx1 was upregulated by BMP9 at the mRNA and protein level in MEFs cells; Ad-Runx1 could enhance the expression of Runx1 at the mRNA and protein level. Runx1 can increase ALP activity and calcium deposition of MEFs cells induced by BMP9,and promote then expressions of Runx2 at the mRNA and protein level. Conclusion： BMP9-induced osteogenic differentiation is partially improved by Runx1 in mesenchymal stem cell line MEFs.