中文摘要：

背景变应原特定的免疫疗法能由调整个人的有免疫力的地位导致有免疫力的忍耐到特定的变应原。然而，它的临床的申请在病人和未确证的安全之中在功效由于单个差别被限制。1,25 Dihydroxyvitamin D3 (1,25 (哦) 2D3 ) 被显示了涉及许多生理的过程，包括有免疫力的反应规定。在现在的学习，我们探索了 1,25 的角色(哦) 为 immunotherapy.Methods 的 2D3 预告的处理七十五只 BALB/c 老鼠随机被划分成五个组(15 只老鼠每组) 。老鼠过敏气喘模型被 ovalbumin 的 intra 腹注射建立(卵， 10 g ) 并且铝氢氧化物(2 mg ) 作为一个助手。1,25 的 50 ng 的 Intra 腹注射(哦) 2D3 用作一个预告的处理，卵(100 g ) 的下的注射作为免疫疗法，和 1% 卵吸入作为挑战。组织病理学说的分析每组在四只老鼠上被执行。在 bronchoalvolar lavage ( BAL )的房间和他们的分类的数字液体是浆液的 assayed.Levels 在 BAL 液体的卵特定的免疫球蛋白 E ( slgE )和 IFN-Y ， IL-4 ， IL-5 和 IL-10 被 ELISA.Results 在 1,25 以后测量(哦) 2D3 预告的处理，当时，免疫疗法能显著地与过敏气喘禁止煽动性的房间的渗入进肺纸巾和老鼠的 BAL 液体与未经治疗的动物相比(嗜曙红血球:( 7.46 (平均( 3g

英文摘要：

Background Allergen-specific immunotherapy can induce immune tolerance to specific allergens by regulating immune status of individuals. However, its clinical application is limited due to individual differences in efficacy among patients and un-confirmed safety. 1,25 Dihydroxyvitamin D3 （1,25（OH）2D3） has been shown to be involved in a variety of physiological processes, including immune response regulation. In the present study we explored the role of 1,25（OH）2D3 pretreatment for immunotherapy.Methods Seventy-five BALB/c mice were randomly divided into five groups （15 mice per group）. The mouse allergic asthma model was established by intra-peritoneal injection of ovalbumin （OVA, 10 μg） and aluminium hydroxide （2 mg）as an adjuvant. Intra-peritoneal injection of 50 ng of 1,25（OH）2D3 served as a pretreatment, subcutaneous injection of OVA （100 μg） as an immunotherapy, and 1% OVA inhalation as a challenge. Histopathological analysis was performed on four mice per group. The number of cells and their classification in bronchoalvolar lavage （BAL） fluid were assayed.Levels of serum OVA-specific immunoglobulin E （slgE） and IFN-Y, IL-4, IL-5 and IL-10 in BAL fluid were measured by ELISA.Results After 1,25（OH）2D3 pretreatment, immunotherapy could significantly inhibit the infiltration of inflammatory cells into lung tissues and BAL fluid of mice with allergic asthma when compared with un-treated animals （eosinophils：（7.46±1.34）×104/ml vs. （13.41±1.67）×104/ml, P ＜0.05）. In addition, levels of IL-4 （（36.g1±7.87） pg/ml vs. （43.70±6.42）pg/ml, P ＞0.05） and IL-5 （（41.97±7.93） pg/ml vs. （60.14±8.35） pg/ml, P ＜0.05） in BAL fluid and serum slgE （（0.42±0.05）vs. （0.75±0.06） OD units, P ＜0.05） were profoundly reduced. However, the IL-10 level in BAL fluid was significantly increased （（67.74±6.57） pg/ml vs. （44.62±8.81） pg/ml, P ＜0.05）.Conclusions These results indicated that 1,25（OH）2D3 pretreatment e