采用电沉积法在玻碳(GC)电极表面合成纳米级聚吡咯(PPy),通过扫描电镜得到PPy的形貌。以PPy为载体,通过吸附法固定葡萄糖氧化酶(GOD),得到GOD/PPy/GC电极。利用循环伏安法对GOD/PPy/GC电极的电化学行为进行分析,结果表明,以PPy为载体可以很好地固定GOD并保持其生物活性。在0.1mol/L磷酸盐缓冲溶液中,无任何电子媒介体存在时,GOD/PPy/GC电极显示了很好的电催化性能。
Nano-polypyrrole(PPy) were prepared on glassy carbon(GC) electrodes by electrodeposited method.The morphology of the PPy was obtained by scanning electron microscopy(SEM).The GOD/PPy/GC electrode was prepared by immobilization glucose oxidase(GOD) on the PPy.The electrocatalytic properties of GOD/PPy/GC electrode were characterized by cyclic voltograme method;the results showed that GOD can be immobilized on the nano-PPy and the biological activation of GOD was retained.The GOD/PPy/GC electrode exhibits good eleccatalytic performance without any electron mediator at the medium of phosphate buffer(0.1mol/L).