中文摘要：

目的：研究Wnt信号传导通路中Wnt1蛋白在口腔鳞状细胞癌（oral squamous cell carcinoma, OSCC）中的表达水平，初步探讨Wnt1在OSCC淋巴结转移中的作用。方法：采用免疫组织化学方法，检测60例OSCC中Wnt1的表达情况；采用Western blot法检测5对OSCC临床标本和配对癌周组织中Wnt1的表达；通过实时定量PCR法和Western blot法分别检测高、低转移潜能的HN-12和HN-4口腔鳞癌细胞系中Wnt1基因和蛋白的表达水平；采用生长曲线、划痕实验和Transwell法，分别检测重组蛋白Wnt1对2种细胞的生长情况、迁移能力和侵袭能力的影响。结果：24例淋巴结转移的OSCC中高表达Wnt1，16例未有淋巴结转移的OSCC中低表达Wnt1，Wnt1的表达在两组之间差异有统计学意义（P〈0.01）；高转移细胞系HN-12较低转移细胞系HN-4在mRNA水平和蛋白水平上Wnt1表达显著增高；重组蛋白Wnt1，能够提高肿瘤细胞的生长、迁移和侵袭能力。结论：Wnt信号通路中的关键分子Wnt1在OSCC中有表达，且与淋巴结转移相关，为进一步探讨Wnt信号通路影响肿瘤转移的分子机制奠定基础。

英文摘要：

Objective： To study whether the expression of Wnt1 in patients with oral squamous cell carcinoma （OSCC） were correlated to lymph node metastasis. Methods： Immunohistochemistry was used to detect Wnt1 expression in OSCC tissues of 60 patients. Western blot was used to detect Wnt1 expression in 5 pairs of OSCC tissues and adjacent normal mucosa tissues . Real-time PCR and Western blot were used to detect Wnt1 expression on mRNA and protein levels re-spectively in HN-12 cells, which was highly metastatic compared with HN-4 cells. Growth curve, scratch test and tran-swell assay were used to detect the growth, migration and invasion ability of HN-12 and HN-4 cells which were treated with or without Wnt1 recombinant protein. Results： Wnt1 protein was up-regulated in 24 cases of OSCC with lymph node metastasis compared to 16 cases without lymph node metastasis （P〈0.01）. Moreover, the expression of Wnt1 on mRNA and protein levels was both much higher in HN-12 cells than HN-4 cells. Lastly, Wnt1 could notably enhanced cell growth, migration and invasion. Conclusion： The high expression of the key member Wnt1 of Wnt signal pathway was re-lated to lymph node metastasis of OSCC.