中文摘要：

AP2/EREBP 抄写因素(TF ) 在植物开发，神经质的规定和压力反应起很重要的作用。在克隆的染色体宽的 cDNA 之上，种系发生并且这的表示模式分析种特定的 TF 家庭，我们包括 At1g71450， At1g50680 和 At5g13910 发现了三个成员，多半响应骆驼毛的织物，寒冷和盐被包含。包含这三 TF 的通常认为的全身的 ORF 的互补直接存储器存取个别地被获得并且熔化到 GAL4 直接存储器存取绑定领域。所有 3 基因有效地工作了 asfrans 使活跃之物使用酵母一个混血儿试金。RT-PCR 实验证明 At1g71450 基因被骆驼毛的织物和低温度导致；At1g50680 基因对许多压力条件，但是特别到冻结温度应答；并且 At5g13910 基因被高盐处理，干旱和乙烯导致。由寻找 ABRC T-DNA 插入异种股票，我们为这些 TF 获得了猛烈的线。同型结合的 ko1 (At1g71450 ) 植物在 stomata 运动在种子萌芽期间并且也显示出过分敏感的回答到骆驼毛的织物。同型结合的 ko2 (At1g50680 ) 植物在在寒心的处理以后与野类型相比冻结忍耐的植物显示出重要减小。同型结合的 ko3 (At5g13910 ) 比野类型植物对高咸度不太容忍。Ourdata，建议 At1g71450 是在骆驼毛的织物发信号的一个否定管理者 At1g50680 和 At5g13910are 在冷、咸的压力回答的积极管理者分别地。

英文摘要：

AP2/EREBP transcription factors （TFs） play very important roles in plant development, hormonal regulation and stress response. Upon genome-wide cDNA cloning, phylogenetic and expression pattern analyses of this plant specific TF family, we found that three of the members including Atlg71450, Atlg50680 and At5g13910, were likely involved in responses to ABA, cold and salt. Complementary DNAs containing putative full-length ORFs of these three TFs were obtained and fused individually to the GAL4 DNA-binding domains. All the 3 genes functioned effectively as trans-activators using yeast one-hybrid assays. RT-PCR experiments showed that the Atlg71450 gene was induced by ABA and low temperature; the Atlg50680 gene was responsive to quite a few stress conditions, but especially to freezing temperature; and the At5g13910 gene was induced by high salt treatment, drought and ethylene. By searching the ABRC T-DNA insertion mutant stocks, we obtained knockout lines for these TFs. Homozygous kol （Atlg71450） plants showed a hypersensitive response to ABA during seed germination and also in stomata movement. Homozygous ko2 （Atlg50680） plants showed a significant reduction in plant freezing tolerance compared to the wild type after chilling treatment. Homozygous ko3 （At5g13910） were less tolerant to high salinity than wild type plants. Our data suggest that At1971450 is a negative regulator in ABA signaling, while Atlg50680 and At5g13910 are positive regulators in cold and salt stress responses, respectively.