中文摘要：

目的研究α-硫辛酸（a—LA）对糖尿病大鼠视网膜病变及血管内皮生长因子（VEGF）表达的影响及机制。方法选择72只健康的雄性Wistar大鼠作为实验材料，将72只大鼠分为4组：空白对照组（A组）12只，糖尿病模型组（B组）24只，α—LA治疗组（C组）24只，葡萄糖干预组（D组）12只，B～D组大鼠按照60mg／kg的剂量腹腔注射链脲佐菌素（STZ），C组用100mg／kg的α—LA灌胃，D组用5．0％的葡萄糖溶液灌胃。比较各组大鼠的体质量、空腹血糖（FPG）、空腹胰岛素（FINS），稳态胰岛素抵抗指数（HOMA-IR）、VEGF表达、超氧化钠歧化酶（SOD）、谷胱甘肽（GSH）和IL-6水平，比较B～D组大鼠的GR分期。结果72h后，B～D组大鼠模型构建成功，A组大鼠FPG为（4．57±0．15）mmol／L，B组为（21．72±4．28）mmol／L，C组为（21．54±4．96）mmol／L，D组为（21．83±4．77）mmol／L，A组与B、C组比较差异有统计学意义（P〈0．05）；A组大鼠体质量（210．5±5．2）g，B组（211．2±5．7）g，C组（209．8±5．8）g，D组（208．7±3．4）g，4组的差异无统计学意义（P〉0．05）。4组大鼠4、8、12周的体质量、FPG、FINS、HOMA—IR、VEGF表达、SOD、GSH和IL-6水平差异有统计学意义（P〈0．05）。12周后，B组、C组和D组大鼠的眼底镜检查GR分期差异有统计学差异（P〈0．05）。结论α—LA的应用可以有效抑制大鼠视网膜VEGF的表达，这一作用可能与改善胰岛素抵抗、抑制慢性炎症和氧化应激多个环节相关。

英文摘要：

Objective To study the effect of α-lipoic acid on the retinal expression level of VEGF and diabetic retinopathy in rats with diabetes mellitus and mechanism. Methods Totally 72 Wistar rats were divided into 4 groups：12 （control group）in group A,24 in modeling group（group B） ,24 in group treated withα-LA（group C） and 12 in high-glucose（group D）. Group B to group D were given 60 mg/kg STZ through intraperitoneal injection,rats in group C were given 100 mg/kg α-LA and rats in group D were given 5.0% glucose-solution. The body mass,FPG,FINS, HOMA-IR,expression level of VEGF, activity of SOD, GSH and IL-6 of 4 groups were compared by statistics. Results After 72 h,the FPG of group A was（4. 57±0. 15）mmol/L,that of group B was （21.72±4.28）mmol/L,that of group C was（21.54±4.96）mmol/L and that of group D was（21.83±4.77）mmol/L,the difference had statistical significance （P;0.05）. The body mass of group A was（210.5 ; 5.2）g, that of group B was （211.2±5.7） g, that of group C was（209.8±5.8）g and that of group D was（208.7±3.4）g,the difference had no statistical significance （P〈0.05）. The body mass,FPG,FINS, HOMA-IR,expression level of VEGF,activity of SOD,GSH and IL-6 among 4 groups at 4 w,8 w and 12 w had statistical difference （P〈0.05）. After 12 w, the difference of GR stage among group B to group D had statistical significance （P〈0.05）. Conclusion α-LA can inhibit the expression of VEGF in rats with diabetes mellitus,which is related to its ability to reduce the oxidative stress and inflammation reaction, as well as to alleviate the insulin resistance.