中文摘要：

目的：探讨一次性向心运动对白介素6（IL-6）抗炎效应的影响,及PGC-1α在其中的角色。方法：2月龄雄性C57BL/6小鼠70只,建立一次性上坡跑运动模型（速度10 m/min,坡度5°）,分别选取运动前（Control）,运动30 min和60 min（E30,E60）,运动恢复期30 min、60 min、3 h和6 h（PE30、PE60、PE3h、PE6h）为实验观测点,每组10只动物。二氯荧光素法检测骨骼肌线粒体活性氧（ROS）生成速率,硫代巴比妥酸法检测骨骼肌MDA含量,荧光定量PCR法检测骨骼肌白细胞介素6（IL-6）、肿瘤坏死因子-α（TNF-α）、过氧化物酶体增殖活化受体γ共激活因子-1α（PGC-1α）和核因子-κB（NF-κB）p65 m RNA表达。结果：与Control组相较,ROS生成速率在E30、E60、PE30、PE60和PE3h组显著升高（P〈0.01）;MDA含量在各组均无显著性变化（P〉0.05）;IL-6 m RNA表达水平在E30、E60和PE30组显著升高（P〈0.05）;TNF-αm RNA表达水平在PE60和PE3h组显著升高（P〈0.01）;PGC-1αm RNA表达水平在E30、E60、PE30、PE60和PE3h组显著升高（P〈0.01）;NF-κB p65 m RNA表达水平在PE60和PE3h组显著升高（P〈0.01）。结论：一次性向心运动可诱导具有抗炎效应的IL-6脉冲式表达升高。PGC-1α可能通过抑制ROS的氧化应激损伤及NF-κB表达两条途径抑制炎症,参与调节向心运动中骨骼肌IL-6的抗炎效应。

英文摘要：

Objective To investigate the impact of an acute concentric exercise on the anti-inflammation of IL-6 and the role of PGC-1α in such process. Methods 70 two-month old male C57BL/6 mice were divided into control group,30-min exercise group（E30）,60-min exercise group（E60）,and 30-min,60-min,3-hour,and 6-hour after exercise groups（PE30,PE60,PE3 h and PE6 h,respectively）. Mice in exercise groups performed a continuous concentric exercise at the speed of 10m/min with 5% slope. Mitochondrial ROS generation rate,contents of MDA,IL-6,TNF-α,PGC-1α,and NFκB-p65 m RNA were determined. Results As compared to the control group,（1）mitochondrial ROS generation rate increased significantly in E30,E60,PE30,PE60 and PE3 h groups（P〈0.01） and the MDA content in all groups remained unchanged（P〈0.05）;（2）IL-6 m RNA level in E30,E60 and PE30 groups,TNF-α m RNA level in PE60 and PE3 h groups,PGC-1αm RNA content in E30,E60,PE30,PE60 and PE3 h groups,and NF-κB p65 m RNA level in PE60 and PE3 h groups increased significantly（P〉0.05 ~P〈0.01）. Conclusion An acute concentric exercise could induce higher impulse-type expression of muscle-derived interleukin-6 mediated by PGC-1α through inhibition of oxidative damage induced by ROS and inactivation of NF-κB.