为了解脱落酸(ABA)缓解小麦幼苗铜胁迫伤害的生理机制,采用营养液培养方法,研究了外源ABA对0.5mmol·L^-1 Cu^2+胁迫下小麦幼苗叶绿素、可溶性糖含量及抗氧特性的影响。结果表明,0.5mmol·L^-1 Cu^2+胁迫对小麦幼苗伤害明显,降低了叶片叶绿素和可溶性糖含量及根、叶片超氧化物歧化酶(SOD)活性,提高了根和叶片丙二醛含量。铜胁迫下,添加1μmol·L^-1 ABA可显著提高小麦幼苗叶片叶绿素和可溶性糖含量及根和叶片过氧化物酶(POD)、叶片SOD活性,降低叶片铜含量。说明适宜浓度的外源ABA可通过调节渗透调节物质含量、增强抗氧化酶活性来减轻铜胁迫对小麦幼苗的伤害,提高其抗铜胁迫能力。
The wheat seedlings were cultivated in Hoagland nutrient solution to study the alleviation effects of abscisic acid(ABA) on wheat seedlings under 0.5mmol·L^-1 Cu^2+ stress. The results showed that 0.5 mmol·L^-1 Cu^2+ stress caused decreases in chlorophyll content, soluble sugar content in leaves and the activity of superoxide dismutase(SOD) in leaves and roots,but an increase in MDA content in the leaves and roots of wheat seedlings. ABA(1μmol·L^-1) applied in the solution increased the contents of chlorophyll and soluble sugar, while decreased the content of copper in leaves. Meanwhile, application of exogenous ABA increased the activity of peroxidase(POD) in leaves and roots and SOD in leaves, while decreased the content of MDA. These results showed that decent concentration of exogenous ABA could reduce the damage under copper stress, and improve the ability of resistance to copper stress in wheat seedlings through regulating the osmotic adjustment substance contents and improving the activity of antioxidant enzymes.