中文摘要：

持家基因一般指在所有细胞中均稳定表达的一类组成型基因，其产物是维持细胞基本生命活动所必需的。持家基因的定义及其发展，反映了其本质和时代特征。本文从基因表达水平量化技术的角度，结合作者利用转录组数据对猪持家基因的分析，讨论了持家基因的定义及其发展，尤其是技术的进步及数据量的剧增对持家基因的定义所引发的问题。本文总结发现，应该在研究技术的进步中，动态地认识持家基因定义所具有的层次性，从而对持家基因的定义不断赋予新的时代特征。

英文摘要：

Housekeeping genes are typically constitutive genes that are required for the maintenance of basic cel- lular functions, and are stably expressed in all cells of an organism under all conditions and during all developmental stages. The definition and development of housekeeping genes reflect their essence and characteristics at different times. The present study reviews the concept development of housekeeping genes based on the technologies that quantify gene expression levels. We also analyze pig housekeeping genes by RNA-seq data, and discuss conceptual issues raised by the rapid progress of technologies and the growth of data. Our present study summarizes that house- keeping genes should be defined at different levels, and changing times will witness its conceptual development.