中文摘要：

植物原生质体作为一个良好的实验系统,广泛应用于植物分子及细胞学研究中.本研究以烟草NC89叶片为材料,采用酶解法制备烟草原生质体,经台盼蓝染色鉴定活性,结果显示,分离纯化后的原生质体产量为（12.7±1.6）×106 g-1,表明该方法能成功地制备高产率、高活力的原生质体,建立了一种烟草原生质体的简易制备方法.

英文摘要：

Abstact：Plant protoplast provides us a unique single cell system in biology study.To explore an effective method of protoplast preparation,we take tobacco leaves as material and digest it in enzyme solution,finally use trypan blue staining to identify the activities of protoplast.The results showed that the yield of protoplasts were up to（12.7±1.6）×106/g,higher than most of the recent reports.It suggest that a method had been established for high-yield and high-activity protoplast preparation in this research which has a certain reference value.