中文摘要：

研究了共存碳源对施氏假单胞菌N2在降解苯酚过程中积累2-羟基黏糠酸半醛（2-HMS）的影响,并用紫外光谱和气相色谱-质谱联用仪（GC-MS）对N2菌的生长过程进行了检测。结果表明,与单一碳源苯酚相比,N2菌在共存碳源甲醇、乙醇、丙酮存在的体系中生长快,且11 h内,苯酚同步降解率分别提高了4.5%、18.5%、16.6%,同时培养液中2-HMS积累量均达到最大。N2菌代谢苯酚可通过羧化反应产生对羟基苯甲酸,也可通过羟化反应产生邻苯二酚,后者通过间位开环裂解生成2-HMS,而甲醇、乙醇、丙酮与苯酚共存时,更易采用后一种代谢途径。

英文摘要：

The article intends to make a study of the effects of the co-existing carbon source on the ring cleavage of phenol by characterizing the Pseudomonas stutzeri N2 and the biochemical mechanism about the phenol biodegradation by N2 in the presence of co-metabolic carbon sources. For our study purpose,we have analyzed the growing process of strain N2 and its biodegradation intermediates by UV spectrum and GC-MS. As compared with the condition of taking phenol as the sole carbon energy source,as a matter of fact,ethyl alcohol or acetone,strain Pseudomonas stutzeri N2 tends to grow faster in the presence of methyl alcohol,whereas the accumulation of 2-HMS,which turned to be medyellow and reach its absorption peak during a period of 375 nm,appeared more noticeable. During a period of 11 h,it would be possible to promote the accumulation of 2-HMS in the growth of media with co-metabolites methyl alcohol,ethyl alcohol or acetoneby 4. 5%,18. 5% and 16. 6%,respectively,and so would it do to the degradation rates of phenol. Since ethyl alcohol is the most efficient co-metabolite for the phenol ring cleavage through catechol and meta-pathway,it is also the most efficient co-metabolite for producing more acidic compounds,which tends to result in the fall of the p H value more obviously. Moreover,the appearance of the absorbance peak at a value of 375 nm tends to confirm the accumulation of 2-HMS as is seen by the change from GCMS. The results from GC-MS also show that the detectable biodegradation intermediates produced by strain N2 have to be catechol,cis,cis-muconate,p-hydroxybenzoic acid,and 2-HMS,too,which should be the main intermediate products with the ethyl alcohol being the co-metabolite. What is more,phenol biodegradation can also be realized by using Pseudomonas stutzeri N2 strain via carboxylation,so as to gain the p-hydroxybenzoic acid,or via hydroxylation to form the catechol,which is by nature a product gained by further transformation into 2-HMS via a metapathway of ring cleavage,or transformed to cis,cis-mucon