中文摘要：

目的：观察缺氧诱导因子-1α（HIF-1α）沉默后人卵巢癌细胞顺铂耐药株SKOV3／DDP的HIF-1α、多药耐药基因1（MDR1）和B细胞淋巴瘤／白血病-2基因（Bcl-2）mRNA及蛋白产物的表达，探讨HIF-1α逆转SKOV3／DDP耐药性的机制。方法：体外培养卵巢癌细胞株SKOV3（敏感组）及其顺铂耐药株SKOV3／DDP（耐药组），部分耐药株转染HIF-1α干扰质粒pshRNA-HIF（转染组）及对照质粒pshRNA—Control（对照组）。RT—PCR法检测各组细胞HIE-1α、MDR1和Bcl-2mRNA表达量；Western blotting和免疫组织化学法测定HIF-1α、P—gP（MDR1基因编码蛋白）和Bcl-2蛋白的表达量。结果：RT—PCR检测，敏感组和转染组HIF-1α、MDR1和Bcl-2mRNA表达量明显低于耐药组（P〈0．05）。Western blotting检测，敏感组和转染组HIF-1α、MDRl和Bcl-2蛋白表达量明显低于耐药组（P〈0．05）；免疫组织化学法，敏感组和转染组HIF-1α、MDR1和Bcl-2蛋白表达量明显低于耐药组（P〈0．05）；MDR1、Bcl-2mRNA及蛋白在敏感组与转染组的表达量差异无统计学意义（P〉0．05）。HIF-1α表达与MDR1、Bcl-2mRNA表达量均呈正相关关系（r=0．908，P=0；r=0．916，P=0）；HIF-1α表达与P—gP、Bcl-2蛋白表达呈正相关关系（r=0．773，P=0．003；r=0．862，P=0）。结论：HIF-1α沉默逆转人卵巢癌细胞顺铂耐药株SOV3／DDP耐药性可能与MDR1和Bcl-2表达降低有关联。

英文摘要：

Objective To observe the expressions of hypoxia inducible factor-1 alpha （HIF-la）, multidrun resistance （MDR1）, and B cell lymphoma/leukemia-2 （Bcl-2） mRNA and protein in human ovarian cancer DDP resistant strain SKOV3/DDP after HIF-1α silencing, and to explore the mechanism of the resistance of SKOV3/ DDP reversed by HIF-1α gene silencing. Methods The ovarian cancer cell strain SKOV3 （sensitivity group） and DDP resistance strain SKOV3/DDP （resistance group） were cultured in vitro and parts of the SKOV3/DDP were transfected with HIF-1α interfere plasmid pshRNA-HIF （transfection group） or control plasmid pshRNA-Control （control group）. RT-PCR method was used to detect the expression levels of HIF-1α, MDR1 and Bcl-2 mRNA;Western blotting and immunohistochemisty methods were used to determine the expression levels of HIF-1α, P-gp （MDR1 gene encoding protein）, and Bcl-2 protein in the cells. Results The expression levels of HIF-1α, MDR1 and Bcl 2 mRNA in sensitivity group and transfection group were significantly lower than those in resistance group （P〈0.05） detected by RT-PCR; the expression levels of HIF-1α, MDR1, and Bcl-2 protein in sensitivity group and transfection group were significantly lower than those in resistance group （P〈0.05） determined by Western blotting and immunohistochemistry; the expression levels of MDR1, Bcl 2 mRNA and protein in sensitivity group and transfection group had no significant difference （P〈0.05）. The expressions of HIF-1α and MDR1 and Bcl -2 were closely related （correlation coefficient ofmRNA, r=0.908, P=0; r=0.916, P=0; correlation coefficient of protein, r= 0. 773, P=0. 003; r 0. 862, P=0）.Conclusion The resistance of ovarian cancer cell SKOV3 / DDP HIF-1α gene silencing may be related to the down-regulation of MDR1 and Bcl 2 expressions.