中文摘要：

目的研究miR．152分子对JEG-3细胞中HIA—G表达水平的调控。方法在滋养细胞肿瘤细胞系JEG-3细胞中分别转染pre-miR-152（实验组），Cy3标记的pre—miRNA—control（阴性对照），空白对照（NC）为未转染的JEG-3细胞。转染48h、72h分别进行RT．PCR与Westernblot实验检测细胞中miR-152、HLA—GmRNA与蛋白的表达水平。结果RT-PCR结果显示在细胞中转染pre—miR-152分子后miR-152的表达显著增高（P〈0．05）；转染后HLA—GmRNA的表达水平在各组之间差异无统计学意义（P〉0．05）。Westernblot结果显示与对照组相比，实验组的HLA—G蛋白表达水平显著降低（P〈0．05）。结论miR-152可能在翻译水平抑制HLA-G的表达。

英文摘要：

Objective To investigate the effect of miR-152 on the expression of HLA-G in JEG-3 cells. Methods The trophoblastic tumor JEG-3 cells were transfeeted with pre-miR-152（as test group）,pre-miRNA-control labeled with dye Cy3 （as negative control） , and with nothing（ normal control, NC） , respectively. 48 and 72 hours after transfction, ceils were used for RT-PCR and Western blot analysis to investigate the expression of miR-152 mRNA and HLA-G mRNA, HLA-G protein, respectively. Resuits After pre-miR-152 transfection, RT-PCR analysis showed that the transfection was effective（ P 〈 0.05 ） , whereas no significant changes were observed in the mRNA level of HLA-G. However, the cells transfected with pre-miR-152 significantly decrease the HLA-G protein level（P 〈 0.05 ）. Conclusion The miR-152 can down-regulate the expression of HLA-G by inhibiting the translation process.