中文摘要：

以谷胱甘肽（GSH）为稳定剂，在水溶液中制备稳定的CdTe纳米量子点。基于溶菌酶在pH7．4的磷酸盐缓冲液中对该量子点的共振散射有增强作用，建立一种简便灵敏的测定溶菌酶的方法。考察了缓冲液pH、量子点的浓度和反应时间等对溶菌酶测定的影响。结果表明，量子点在波长422nm处的散射光强度的增强与溶菌酶浓度呈线性关系，线性范围为1．4—28mg／L，检出限0．027mg／L（3σ）。此方法应用于溶菌酶含片中溶菌酶含量的检测，回收率在98．7％-98．9％之间，相对标准偏差〈4．2％。

英文摘要：

CdTe quantum dots （QDs） were synthesized by chosing L-Glutathione （GSH） as modifying agent in aqueous solution. Based on the significant enhancement effect of lysozyme on the resonance light Scattering GSH-CdTe in the phisphate buffer at pH 7.4, a convenient and feasible method for the determination of lysozyme was proposed. The effects of experimental conditions,including pH of the buffer, the concentration of CdTe QDs and the reaction time on the determination of lysozyme were investigated. The results showed the enhanced RLS intensity of CdTe QDs was linear with mass con- centration of lyszome in the range of 1.4 - 28 mg/L as measured at 422 nm, with the detection limit of 0. 027 mg/L （3or）. The method was used to the detection of the lysozyme in lyszome buccal tables samples with the recovery from 98.7% -98.9% and the RSD lower than 4. 2%.