中文摘要：

目的：观察C57BL／6小鼠肝脏中NK细胞、T细胞和NKT细胞的含量、亚型和细胞因子产生能力。方法：分离正常C57BL/6小鼠肝脏的淋巴细胞，通过细胞表面分子染色以及流式细胞术（FCM）检测NK1．1^＋NK细胞、CD3^＋T细胞和CD3^＋NK1．1^＋NKT细胞的含量及其表面CD4、CD8分子的表达情况；细胞经过PMA＋Ionomycin刺激后，应用细胞内细胞因子染色并结合FCM检测NK1．1^＋NK细胞、CD3^＋T细胞和CD3^＋NK1．1^＋NKT细胞产生IFN-γ、IL-4、IL-9和IL-17细胞因子的情况。结果：肝脏中NKT细胞的含量为（21．51±13．02）％，高于NK细胞和T细胞（P〈0．05）。NK细胞以CD4^-CD8^-亚群为主，T细胞和NKT细胞均以CD4^＋细胞亚群为主。NKT细胞分泌的IL4的比例高于NK细胞和T细胞（P〈0．05）。T细胞分泌IL-17的能力明显强于NK细胞和NKT细胞（P〈0．05），IL-9^＋细胞在三种细胞中含量不高（〈0．03％）。结论：C57BL/6小鼠肝脏中NK细胞、T细胞和NKT细胞含量、表型和功能方面可能存在明显的差异。

英文摘要：

Objective：To observe the content, subtype and production ability of NK, T and NKT cells in the livers of C57BL/6 mice. Methods： The lymphocytes were isolated from the livers of normal C57BL/6 mice. The surface molecule staining and flow cytometry （ FCM ） were used to detect the contents of NK1.1^＋ NK, CD3^＋ T and CD3^＋ NKI. 1^＋ NKT and expressions of CD4 and CD8 on their surfaces. After stimulation of PMA^＋ Ionomycin, the intracellular cytokine staining combined with FCM was used to detect the productions of IFN-γ, IL-4, IL-9 and IL-17 by NK1. 1^＋ NK, CD3^＋ T and CD3 ＋ NK1. 1 ＋ NKT cells. Results： In the liver, the content of NKT cells was （ 21.51 ＋ 13.02 ） %, which was significantly higher than that of the NK and T cells （ P 〈 0.05 ）. The CD4 - CD8 - subgroup was mainly in the NK cells, while the CIM^＋ subgroup was mainly in both the T and NKT cells. The proportion of IL-4 secreted by NKT cells was higher than that of NK and T cells （ P 〈 0. 05 ）. The IL-7 secretion ability of T cells was significantly higher than that of NK and NKT cells （ P 〈 0.05 ）. However, the content of IL-9^＋ cells was not high in these three cells （ 〈0.03% ）. Conclusion： There might be significant difference in content, phenotype and function in NK cells, T cells and NKT cells from the livers of C57BL/6 mice.