中文摘要：

目的观察微重力生物反应器内拟胚体（EBs）来源细胞的肝细胞分化与成熟。方法将未分化鼠胚胎干细胞（ES细胞）以1×10^6/mL移入微重力反应器内,在DMSO、地塞米松、FGF4、HGF等刺激作用下,进行15 d的旋转培养。采用ELISA法动态检测培养液中鼠白蛋白的产生量。培养结束时转移EBs于载玻片和培养板,分别检测EBs来源细胞的糖原储存和对靛青绿（ICG）、荧光素标记低密度脂蛋白（D iI-Ac-LDL）的摄取能力。结果在生物反应器旋转培养的第5、10 d,未从培养液中检出特异的鼠白蛋白,而在第15 d的培养液中检出一定量的鼠白蛋白。与原代培养的成熟肝细胞一样,转种的EBs来源细胞period ic ac id-Sch iff（PAS）糖原染色阳性,ICG摄取试验阳性,D iI-Ac-LDL摄取试验阳性。未分化的ES细胞均呈阴性。结论微重力生物反应器不仅能加快EBs的形成与肝细胞分化,而且能促进分化肝细胞的成熟。

英文摘要：

Objective To investigate the differentiation and maturation of embryoid bodies（EBs） derived cells in a microgravity bioreactor.Methods Undifferentiation mouse embryonic stem cells（ES cells） at a final concentration of 1×106/mL of medium were seeded into a rotary microgravity bioreactor for a 15 days＇ culture with IMDM medium containing DMSO,dexamethasone,fibroblast growth factor 4,hepatic growth factor,etc.Mouse albumin,produced by EBs derived cells,were examined by ELISA.Meanwhile,the glycogen store,indocyanine green（ICG） uptake and low-density-lipoprotein uptake by EBs derived cells were examined.Results Mouse albumin were not detected at day 5 and 10 culture medium by a specific ELISA kit,but were detected at day 15 culture medium.As primary cultured and mature mouse hepatocytes,EBs derived cells were positive for periodic acid-Schiff staining,ICG uptake and DiI-Ac-LDL uptake.The undifferentiation mouse ES cells were negative for these staining and uptake tests.Conclusion The application of the simulating microgravity bioreactor can not only accelerate the EBs formation and hepatic differentiation,but also promote the maturation of differentiated hepatocytes.