中文摘要：

目的：探讨LMP1对鼻咽癌中转录因子c-Jun和Ets1表达的影响，以及对Ets1和AP-1间相互作用的调控，为LMP1的致瘤机制提供新的依据。方法：选用可调控表达LMP1的鼻咽癌细胞系pTet-on-LMP1 HNE2（L7细胞），通过针对c-Jun、Ets1的硫代反义寡核苷酸进行阻断，观察LMP1对c-Jun、Ets1表达的影响及其相互作用，蛋白质印迹法检测Ets-1、c-Jun蛋白质表达。免疫共沉淀（IP）结合Western blot研究c-Jun和Ets1相互结合的情况。结果：在不同浓度Dox诱导24h后，Dox为0．6wg／mL时的c-Jun和Ets1表达均最强。随着Dox诱导时间的延长，L7细胞中c-Jun和Ets1的表达上调，至4h达到最高。阻断c-Jun表达后，Ets1表达显著降低；阻断Ets1表达后，c-Jun表达显著降低。在Dox诱导的L7细胞总蛋白中，在口沉淀的Ets1中存在c-Jun表达，并且在Dox 0．6μg／mL时最强；在口沉淀的c-Jun中存在Ets1表达，同样在Dox 0．6μg／mL时最强。结论：EBV-LMP1可以调控转录因子c-Jun和Ets1表达，且在调控过程中可能存在c-Jun和Ets1间的相互作用。

英文摘要：

Objective： To investigate the effect of EBV encoded latent membrane protein1 （LMP 1 ） on AP-1 and Ets 1 expression, and the interaction between c-Jun and Ets1. Methods： NPC cell line, pTet-on-LMP1 HNE2 （L7 cell）were used in which LMP1 expression regulated by Doxycycline （Dox）. To confirm the effect of LMP1 on Ets1 and c-Jun, protein expression of Ets1 and c-Jun in L7 cells were assayed by Western Blot with blockade of c-Jun or Ets 1 antisense oligonucleotides. We further discussed the effect of LMP 1 on the interaction between c-Jun and Ets1 with antisense blocking and Western blot. To observe if the interaction of c-Jun with Ets1 results in the formation of complex, we combined co-immunoprecipitation with Western blot. Results： The expression of c-Jun and Ets1 showed dose-effect relationship and were higher after induced by 0.6μg/mL Dox than by other dose for 24 h. It is showed a time-effect relationship and the c-Jun and Ets1 expressions were higher induced by 6μg/mL Dox for 4h. Under the Dox induction, Ets1 expression was inhibited significantly after the blocking of c-Jun; c-Jun expression decreased greatly after the blocking ofEts1. After Ets-1 protein was precipitated with anti-Ets-1-antibody, c-Jun expression showed positive in the Ets1 protein precipitated; on the contrary, after c-Jun protein was precipitated with anti-c-Jun-antibody, Ets1 expression showed positive in the c-Jun protein precipitated, suggesting that the complex may be formed between c-Jun and Ets1. Conclusion： This suggested that LMP1 could have a great effect on c-Jun and Ets1 expression and the interaction between c-Jun and Ets1.