中文摘要：

目的：观察鼻咽癌病人树突状细胞（DC）经体外分离诱导分化后表型变化和呈递功能的情况。方法：在体外分离并诱导成熟HLA—A2基因型鼻咽癌病人自身的DC，运用流式细胞仪检测DC的表型变化，然后分别负载EB病毒抗原LMP-2的HLA—A2限制型两个表位肽段CLGGLLTMV（CLG）和LTAGFIFL（LTA），诱导自身的CD8^＋T细胞。培养两周后，运用酶联免疫斑点法（ELISPOT）检测经DC诱导后CD8^＋T细胞的免疫应答情况。结果：分离鼻咽癌患者外周血DC，培养7d后用流式细胞仪检测DC的各项表型：CD80、CD86、CD83、HLA-DR等，呈现分化成熟的表型特征。上述各种表型的细胞百分率分别为（33．92±21．42）％、（90．20±12．10）％、（32．89±23．47）％、（90．37±12．82）％，并且与正常人的DC的表达无明显差异。培养成熟的DC呈递肽段CLG及LTA给自身CD8^＋T细胞，T细胞识别肽段并能应答的细胞数增加，呈递前分别为（2．67±1．97）2×10^4CD8^＋T细胞和（5．33±1．86）2×10^4CD8^＋T细胞，呈递后为（42．67±33．79）2×10^4CD8^＋T细胞和（25．67±18．25）2×10^4CD8^＋T细胞，呈递前、后比较差异有统计学意义（P〈0．05）。同时与正常T细胞的应答能力无明显差别。结论：鼻咽癌病人外周血DC可经外周血分离培养，诱导扩增后能分化成熟并能有效呈递肿瘤抗原肽段，产生特异性CTL，对鼻咽癌病人的免疫治疗具有潜在的应用价值。

英文摘要：

Objective：To study the change of the epitope and function of dendritic cell in peripheral blood from Nasopharyngeal carcinoma patients. Methods： DCs were isolated from HLA-A2 peripheral blood of NPC patients and cultured with cytokine. Membrane marks of DCs were detected by flow cytometry after 7-day culture. Then DCs were stimulated by peptides CLGGLLTMV （CLG） and LTAGFIFL（LTA）that are the HLA-A2 restricted epitope peptides from LMP-2 protein. After 2-week culture, autologous CD8^＋ T was pulsed with the DCs, and the frequency of CD8^＋ T cells that secret IFN-garmma in response to the DCs were detected in Elispot assay. Result： The percentages of CD80^＋ DCs, CD86^＋ DCs, CD83^＋ DCs and HLA-DR^＋ DCs were （33.92±21.42）% ,（90. 20±12.10）% ,（32. 89±23.47）% and（90.37±12.82）% respectively, and there was no difference compared with the normal control group. The number of T cells that can recognize and respond to the peptides CLG and LTA are （2.67± 1.97）per 2 × 10^4 CD8^＋ T cells and （5.33±1.86） per 2× 10^4 CD8^＋ T cells before their presence, and after their presence are （42.67±33.79） per 2× 10^4 CD8^＋ T cells and （25.67±18. 25） per 2 × 10^4 CD8^＋ T cells. There are differences compared with pre-presence and pro-presence （ P 〈0.05）. But there are not any difference compared with the normal control group. The result shows the DCs have the ability to present the peptides CLG and LTA to CD8^＋T cells. Conclusion：The DCs in NPC patients can be separated and cultured from peripheral blood. After cultured in mature, DCs loaded with the epitope peptides of LMP-2 from EBV can induce the CD8^＋ T cell to cytotoxic T lymphocyte （CTL）, and this result is very useful for immunological therapy for NPC.