从基于cDNA-AFLP分析的辣椒应答疫霉侵染的表达谱中找到1个特异表达基因的序列;利用这个序列设计引物,采用基于PCR技术的96孔文库筛选方法,从疫霉处理的辣椒cDNA文库中筛选出这个应答辣椒疫霉侵染的候选基因的全长cDNA;经生物信息学分析,推测这个长度为1022 bp的候选基因为辣椒叶绿素a/b结合蛋白(chlorophyll a/b binding protein,cab)基因;并利用荧光定量PCR法检测cab在疫霉侵染下的表达情况。
A sequence of specific expression gene was found in the expression profile responding to infected Phytophthora capsici in popper based on eDNA-FALP analysis, and the sequence was used to design the primer, one positive candidate resistant gene eDNA was isolated from the pepper eDNA library by PCR-based 96-hole screening. According to the results of bioinformafies analysis, it was estimated that the candidate resistant gene with the length of 1022 bp was chlorophyll a/b binding protein gene.