中文摘要：

目的探究apelin-13对离体Wistar大鼠心脏缺血再灌注后的作用效果及机制。方法55只大鼠随机分为五组：正常组（N组）、apelin-13＋正常组（A组）、缺血再灌注组（I-R组）、apelin-13＋缺血再灌注组（A／I．R组）及经典后处理组（I．Post组）。N组持续正常液灌流150min；apelin组给予含apelin-13（500nmol／L）正常液持续灌注150min；I-R组正常液稳定灌流30mill后，结扎前降支30-rain，继以正常液再灌注90min；A／I．R组给予含apelin-13（500nmol／L）正常液再灌注20min，继而以正常液灌流70min；I-Post组再灌注前行经典后处理（30S再通30S缺血，共3次循环）。记录分析左心室发展压（LvI）P）、再灌注心律失常（RA）、心肌梗死面积、各组L型钙通道蛋白ctlc亚单位及钠通道蛋白Vet亚单位的表达水平。结果（1）与N组相比，给予apelin后（即A组）LVDP明显增高（P〈O．05），而进行缺血再灌注处理后LVDP均持续下降，但apelin．13及后处理均可减缓其降低，二者对LVDP的影响差异无统计学意义。（2）对RA的影响：A／I-R组较I-R组RA评分降低（P〈O．05），A／I-R组与I-Post组间RA评分差异无统计学意义（P〉0．05）。（3）对心肌梗死面积的影响：A／I-R组心肌梗死面积较I-R组减小（P〈O．05），A／I-R组与I-Post组间心肌梗死面积差异无统计学意义（P〉O．05）。（4）组间心肌钠、钙通道蛋白表达无差异。结论在心肌缺血再灌注时apelin-13可减少再灌注损伤，改善缺血再灌注后心脏泵功能，减少再灌注性心律失常的发生，缩小梗死面积。apelin-13再灌注具有与经典后处理相似作用，但其作用机制并不是通过改变心肌钠、钙通道蛋白的表达来实现的。

英文摘要：

Objective To observe the effects and mechanism of apelin-13 to ischernia reperfusion through establishing Langendorff rats model. Methods Wistar male rats were randomly divided into 5 groups： normal group （N）, apelin-13 group （A）, ischemla reperfusion group （I-R）, apelin-13＋I-R group （A/I-R） and classical ischemia post conditioning group （I-post C）. N group： continued perfusion for 150 win with KH liquid. A group： continued perfusion for 150 min with KH liquid containing Apelin-13 （500 nmol/L）. I-R group： stable perfusion for 30 rain followed by ligating LAD 30 rain, then reperfusion for 90 rain. A/I-R group： reperfusion with apelin-13 （500 nmol/L） for 20 rain. Then perfused with KH liquid for 70 rain. I-post C group： I-Post C （reperfusion for 30 s, ligation for 30 s, and 3 cycles in all） before reperfusion. Recorded and observed R.A, LVDP by 16 physiological recorder and calculated myocardial infarct size. Observed the expression of myocardial Na＋ channel protein and voltage-gated Ca2＋ channel protein with Western blot. Results About the LVDP： compared with N group, LVDP was significantly increased in apelin-13 administration group （A group）（P〈O.05）, but a steady decline was arisen in groups which treated with I-R. The depression was attenuated by apelin- 13 and I-Post C. A/I-R and I-Post C group had no significant difference in LVDP at the same time （P〉0.05）. About RA score： RA score in A/I-R group was lower than I-R group （P〈0.05）, A/I-R and I-Post C group had no significant difference in RA score （P〉0.05）. About myocardial infarct size： myocardial infarct size in A/I-R was smaller than I-R group （37.45±3.53 vs. 54.27＋3.88, P〈0.05）, A/I-R and I-Post C group had no significant difference in myocardial infarct area （P〉0.05）. The expression of myocardial Na^2＋ channel protein and voltage-gated Ca^2＋ channel protein had no significant difference （P〉0.05）. Conclusions Apelin-13 has the positive inotropic eff