中文摘要：

目的建立UPLC法同时测定桑白皮Mori Cortex30%、50%、80%乙醇组分中桑皮苷A、桑辛素M-6,3＇-O-β-D-二葡萄糖苷、5,7-二羟基香豆素、东莨菪亭、桑辛素M、桑辛素M-3＇-O-β-D-葡萄糖苷、桑黄酮G、桑辛素的含有量。方法分析采用Dionex AcclaimTMRSLC 120 C18色谱柱（2.1 mm×100 mm,2.2μm）;以0.1%乙酸水溶液（A）-甲醇（B）（30%乙醇）、0.1%磷酸水溶液（A）-甲醇（B）（50%乙醇）、0.1%磷酸水溶液（A）-乙腈（B）（80%乙醇）为流动相,梯度洗脱;体积流量0.3 m L/min;柱温20℃;检测波长305 nm（30%乙醇）、315 nm（50%乙醇）、280 nm（80%乙醇）。结果 8种成分均呈良好的线性关系（r〉0.999 0）,平均加样回收率95%~105%,RSD均小于5%。结论该方法准确简便,灵敏度高,适用于桑白皮中化学成分的测定。

英文摘要：

AIM To establish a UPLC method for simultaneously determining the contents of mulberroside A, moracin M-6, 3＇-di-O-13-D-glucopyranoside, 5, 7-dihydroxycoumarin, scopoletin, moracin M, moracin M-3＇-O- [3-D-glucopyranoside, kuwanon G and moracin in 30% , 50% and 80% ethanol extracts of Mori Cortex. METH- ODS The analysis was performed on Dionex AcclaimTM RSLC 120 Cls column （2. 1 mmx 100 ram, 2.2 μm） , mobile phases were 0. 1% acetic acid aqueous solution （A） -methanol （B） （30% ethanol）, 0. 1% phosphoric acid aqueous solution （A） -methanol （B） （50% ethanol） and 0. 1% phosphoric acid aqueous solution （A） - acetonitrile （B） （80% ethanol） for gradient elution, flow rate was 0.3 mL/min, column temperature was main- tained at 20 ~C, and detection wavelengths were set at 305 nm （30% ethanol）, 315 nm （50% ethanol） and 280 nm （80% ethanol）. RESULTS These eight constituents showed good linear relationships （r 〉 0. 999 0）, whose average recoveries were 95% - 105% with the RSDs of less than 5%. CONCLUSION The method is ac- curate and simple with high sensitivity, which is suitable for the determination of chemical constituents in Mori Cortex.