氰化物为剧毒物质,针对氰化物研究出一种快速、可视化检测方法是非常有必要的。研究发现,荧光分子Singapore Tongue(SGT-5)与Hg2+的混合体系(命名为Cyanogen Red,CNR)可以作为新的荧光传感器,对饮用水规定安全阈值内的CN-实现快速、可视化的检测。当水体中CN-浓度为0.210μmol/L时,CNR的荧光值与CN-浓度呈现良好线性关系(R2=0.998),并且肉眼可见其荧光红色随CN-浓度增大呈渐暗趋势。CNR对水体中常见的阴离子如Cl-,SO42-,CO32-,HCO3-,NO3-和PO4-单一体系和混合体系都具有选择性。通过对14种实际饮用水样中滴加10μmol/L CN-的准确测定,得出该方法相比较传统硝酸银滴定法具有许多优势,不但需样量少(100μL)、耗时短(1 min),而且检测限完全能够满足安全引用水中CN-的最大允许浓度1.9μmol/L。
The paper is intended to present a novel method for quick and visual detection of the cyanide content in the drinking water known as Cyanogen Red (CNR) by way of combining the fluorescent molecules of Singapore Tongue (SGT -5) with Hg2+ . However, the traditional CN- detection method remains low efficient and time-consuming. In answering to this badly need, we have developed a method known as the quick, visualized testing method for testing the intensity of CNR fluorescence in the drink- ing water, which can help to make clear the tendency of the linear dependence On the concentration of CN - in the deionized water e- ven with the concentration of CN - being smaller than 10μmol/L. Furthermore, by irradiating CNR and CN- mixtures under 365 nm UV lamp, CN - can reduce the red color of CNR to a great extent and be visualized by the naked eyes. As a result, CNR fluorescent method shows a feature of being highly selective among the common anions present in the water containing CI- , SO42- , CO3- , HC03- , NO3- and PO4 , regardless of the single anion or the mixed system. What is more, adding 10 μmol/L CN- to the 14 kinds of real water samples, we have found that CNR fluorescent method tends to be more accurately measurable than it can be done with the traditional CN- detection method. Besides, the newly developed CNR detection method by us proves to be more advantageous, for example, the samples determined by the newly developed method are much smaller in size (only 100μL) , determining much faster (1 min) and lower in detection minimal limits. In addition, it is capable to meet the need of the maximal concentration of 1.9μmol/L CN- as is demanded in the safetyuse drinking water.