中文摘要：

目的：构建βig-h3基因真核表达载体pEGFP-C2／βig-h3并转染人7721肝癌细胞，检测转染后细胞MMPs表达水平的变化。方法：用RT-PCR方法获得βig-h3基因，以pEGFP-C2为载体，构建重组表达质粒pEGFP-C2／βig-h3。重组质粒用脂质体转染人7721肝癌细胞，明胶酶谱法检测转染后细胞MMPs表达水平的变化。结果：正确构建了pEGFP-C2／βig-h3重组质粒，并且在人7721肝癌细胞达到高转染效率，转染后细胞MMPs表达水平明显升高。结论：成功构建了pEGFP-C2／βig-h3真核表达质粒，βig-h3促进人7721肝癌细胞分泌MMPs，提示βis-h3与肝癌的侵袭和转移密切相关。

英文摘要：

βig-h3 was first identified as a transforming growth factor-betal-inducible gene in human lung adenocarcinoma cell line. It encodes for a secreted extracellular matrix （ECM） protein, which is thought to act on cell attachment and ECM composition. Previous study showed that βig-h3 were highly expressed in human hepatoma cell lines and lowly expressed in human normal hepatic cells. The present study aimed to transfect βig-h3 Matrix metalloproteinase Hepatoma cell Eukaryotic expressionig- h3 into 7721 cells to investigate its effect on secretion of MMPs in the transfected human hepatoma cells. Full- length βig-h3 Matrix metalloproteinase Hepatoma cell Eukaryotic expressionig-h3 gene, cloned by reverse transcription polymerase chain reaction （RT-PCR） was inserted into the eukaryotic expression vector pEGFP-C2. The recombinant plasmid was transfected into 7721 cells with Lipofectamine2000 and Gelatin-Zymography were adopted to detect the production of MMPs in the transfected cells. Results showed that βig-h3 Matrix metalloproteinase Hepatoma cell Eukaryotic expressionig-ha/pEGFP-C2 recombinant expression plasmid was successfully constructed and achieved high transfection efficiency. MMPs expression of the transfected cells was promoted significantly. These results suggest that overexpression of βig-h3 Matrix metalloproteinase Hepatoma cell Eukaryotic expressionig-h3 promoted the production of MMPs, indicating that βig-h3 Matrix metalloproteinase Hepatoma cell Eukaryotic expressionig-h3 may play roles in the invasive and metastatic processes of hepatoma.