目的:研究在不同温度条件下家兔死后心血氧化还原电位(oxidation reduction potential,ORP)值变化与死亡时间(postmortem interval,PMI)的关系。方法随机将48只家兔分为6组,以空气栓塞法处死后,取家兔右心室血液分别置于10、15、20、25、30和35℃温度下的水浴中,自取血即刻(0 h)至死后132 h内,每4 h用PB-21型电化学分析仪测定样本的ORP值。应用SPSS 17.0软件建立曲线回归方程,再应用MATLAB 7.10.0软件建立曲面方程和三维曲面图。结果不同温度条件下家兔心血ORP值变化与PMI具有高度相关性,温度高时,ORP值上升明显,温度低时,ORP值上升缓慢,并获得拟合的曲面方程以及三维曲面图。结论拟合的ORP值与PMI的曲面方程及三维曲面图可进行温度变化条件下的PMI推断。
Objective To investigate correlation between the changes of oxidation reduction potential (ORP ) values of heart blood in rabbits after death and postm ortem interval (PMI) at different temperatures. Meth-ods Forty-eight rabbits were random ly divided into 6 groups and sacrificed by air em bolism . Blood sam-ples were taken from the right ventricle of each rabbit and stored at different temperatures of 10, 15, 20, 25, 30and 35℃, respectively. Every 4 hours from 0h to 132 hpostm ortem , the ORP values of the blood samples were measured at different intervals by PB-21 electrochem ical analyzer. The curvilinear regres-sion equation was established by SPSS 17.0software. The surface equation and 3D surface diagram were established by MATL A B7.10.0software. Results The ORP values at different temperatures of heart blood in rabbits were highly correlated with the PMI. The ORP values rised obviously when the temper-ature was high and rised slowly when the temperature was low. The surface equation and 3D surface diagram were obtained. Conclusion The surface equation and 3D surface diagram of ORP values and PMI may be used for PMI estimation at different temperatures.