中国冷杉木(Cunninghamia lanceolata (兰姆) 钩) 是在中国为木材生产使用的最重要的具球果的树种类之一。这里,我们进行了顺序相关的放大多型性(SRAP ) 有 594 教材联合的一个总数的教材屏蔽试金,用 22 在四代表性的中国人上提交, 27 逆行教材冷杉木遗传型。获得的结果显示中国冷杉木 genomic DNA 穿上著名扩大偏爱采用了前面或反向的教材核苷酸(3 个选择底) 。从测试教材集合,与清楚地著名的乐队一起的 35 教材联合,稳定的扩大,和富人,多型性被选择并且鉴别同样最佳的教材集合。这些最佳的教材对给了 379 个 scorable 乐队的一个总数,包括 265 个多态的乐队,与每教材联合的 10.8 个乐队和 7.6 个多态的乐队的一般水准。为每个最佳的教材集合的生产乐队数字与从 33.3 ~ 100.0 跨越的多态的乐队的一个百分比从 7 ~ 14 ?% 。这些教材联合能在中国冷杉木便于下一 SRAP 分析试金。
Chinese fir(Cunninghamia lanceolata(Lamb.)Hook) is one of the most important coniferous tree species used for timber production in China. Here, we conducted a sequence-related amplified polymorphism(SRAP) primer screening assay with a total of 594 primer combinations,using 22 forward and 27 reverse primers on four representative Chinese fir genotypes. The obtained results indicated that Chinese fir genomic DNA has a notable amplification bias on the employed forward or reverse primer nucleotides(30selection bases). Out of the tested primer sets, 35 primer combinations with clearly distinguished bands, stable amplification, and rich polymorphism were selected and identified as optimal primer sets. These optimal primer pairs gave a total of 379 scorable bands,including 265 polymorphic bands, with an average of 10.8bands and 7.6 polymorphic bands per primer combination.The produced band number for each optimal primer set ranged from 7 to 14 with a percentage of polymorphic bands spanning from 33.3 to 100.0 %. These primer combinations could facilitate the next SRAP analysis assays in Chinese fir.