Pentatricopeptide 重复(PPR ) 蛋白质,包含双人脚踏车重复退化 35 个氨基酸主题,为 post-transcriptional 是重要的叶绿体基因表示。在这研究,我们报导颜料缺乏的异种的描述 1 (pdm1 ) 在 Arabidopsis,哪个显示白化体显型。PDM1 包含主题列在后面由的 5 PPR 一请领域。编码质体的聚合酶依赖者叶绿体基因的层次戏剧性地被减少,而那些在异种编码原子核的聚合酶依赖者叶绿体基因增加了。另外, rpoA pre-mRNA 的模式被改变, rpoA 抄本在 pdm1 是不在的。因此,这些结果建议 PDM1 为在 Arabidopsis rpoA pre-mRNA 处理被要求。
Pentatricopeptide repeat (PPR) proteins, containing tandem repeats of degenerate 35 amino acid motifs, are important for post-transcriptional chloroplast gene expression. In this study, we report the characterization of a pigment-deficient mutant 1 (pdml) in Arabidopsis, which displays the albino phenotype. PDM1 contains 5 PPR motifs followed by a PLS domain. The levels of plastid-encoded polymerase-dependent chloroplast genes were reduced dramatically, whereas those of nucleus-encoded poly- merase-dependent chloroplast genes increased in the mutant. In addition, the pattern of rpoA pre-mRNA was altered and the rpoA transcript was absent in pdml. Thus, these results suggest that PDM1 is required for processing of rpoA pre-mRNA in Arabidopsis.