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The PPR protein PDM1 is involved in the processing of rpoA pre-mRNA in Arabidopsis thaliana
  • ISSN号:1001-6538
  • 期刊名称:科学通报(英文版)
  • 时间:0
  • 页码:3485-3489
  • 语言:中文
  • 分类:Q78[生物学—分子生物学] Q943[生物学—植物学]
  • 作者机构:[1]School of Life Sciences, Lanzhou University, Lanzhou 730000, China, [2]Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • 相关基金:This work was supported by the National Basic Research Program of China (2006CB910300) and the National Natural Science Foundation of China (30725003). We would also like to thank the Arabidopsis Biological Resource Center for providing the seeds of Arabidopsis.
  • 相关项目:光合作用及固氮
中文摘要:

Pentatricopeptide 重复(PPR ) 蛋白质,包含双人脚踏车重复退化 35 个氨基酸主题,为 post-transcriptional 是重要的叶绿体基因表示。在这研究,我们报导颜料缺乏的异种的描述 1 (pdm1 ) 在 Arabidopsis,哪个显示白化体显型。PDM1 包含主题列在后面由的 5 PPR 一请领域。编码质体的聚合酶依赖者叶绿体基因的层次戏剧性地被减少,而那些在异种编码原子核的聚合酶依赖者叶绿体基因增加了。另外, rpoA pre-mRNA 的模式被改变, rpoA 抄本在 pdm1 是不在的。因此,这些结果建议 PDM1 为在 Arabidopsis rpoA pre-mRNA 处理被要求。

英文摘要:

Pentatricopeptide repeat (PPR) proteins, containing tandem repeats of degenerate 35 amino acid motifs, are important for post-transcriptional chloroplast gene expression. In this study, we report the characterization of a pigment-deficient mutant 1 (pdml) in Arabidopsis, which displays the albino phenotype. PDM1 contains 5 PPR motifs followed by a PLS domain. The levels of plastid-encoded polymerase-dependent chloroplast genes were reduced dramatically, whereas those of nucleus-encoded poly- merase-dependent chloroplast genes increased in the mutant. In addition, the pattern of rpoA pre-mRNA was altered and the rpoA transcript was absent in pdml. Thus, these results suggest that PDM1 is required for processing of rpoA pre-mRNA in Arabidopsis.

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