通过对不同化工厂废水处理池的活性污泥进行富集、不同浓度银离子驯化后,分离得到30株银离子抗性细菌,最大硝酸银耐受量可达80mg·ml^-1.对这些细菌进行质粒抽提与鉴定,质粒检出率为76.67%.40mg·ml^-1的苯甲酸钠对HAg4细菌质粒的消除率可达98.75%;而350μg·ml^-1的吖啶橙对HAg4细菌质粒的消除率只有77.78%.细菌质粒与银离子抗性密切相关.
Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag^+ solution, thirty bacteria strains with silver (Ag^+)-resistance were isolated, among which, the highest Ag^+ -resistant concentration was 80 mg·ml^-1, The plasmids in these bacteria were extracted, with the detection rate of 76.67 %. The elimination rate of the plasmid in HAg4 bacteria was 98.75 % by 40 mg·ml^-1 sodium benzoate, and 77.78 % by 350 μg·ml^-1 acridine orange. It was suggested that the Ag^+ -resistance of bacteria was highly correlated with their plasmids.