中文摘要：

目的 探讨臭氧对骨性关节炎大鼠关节软骨Wnt/β-连环蛋白（β-catenin）信号通路的影响.方法 健康雄性SPF级Wistar大鼠18只,3月龄,体重200～ 250 g,采用随机数字表法分为3组（n=6）：正常对照组（C组）、骨性关节炎组（OA组）和臭氧组（O组）.采用膝关节腔内注射含3mg碘乙酸钠水溶液50 μl的方法制备骨性关节炎模型.注药后7d,O组右后肢膝关节腔注射25μg/ml臭氧1 ml,每周1次,连续3周.造模后观察大鼠行为学改变.于造模前1d、造模后1、4、7、14、21和28 d时测定机械缩足反应阈.造模后28 d时,截取整个膝关节,行HE染色,光镜下观察软骨病理学结果,采用免疫组化法测定膝关节软骨β-catenin和基质金属蛋白酶-13（MMP-13）的表达水平.结果 大鼠造模后出现跛行、膝关节肿胀、关节活动降低等关节炎的表现,以及舐足等痛觉过敏表现.与C组比较,其余2组造模后各时点机械缩足反应阈降低,膝关节软骨β-catenin和MMP-13表达上调（P＜0.05）;与OA组比较,O组造模后7-28 d时机械缩足反应阈升高,膝关节软骨β-catenin和MMP-13表达下调（P＜0.05）.O组膝关节软骨病理学损伤较OA组减轻.结论 臭氧减轻大鼠骨性关节炎的机制可能与抑制关节软骨Wnt/β-catenin信号通路激活有关.

英文摘要：

Objective To investigate the effects of ozone （O3） on Wnt/β-catenin signaling pathway in the articular cartilage of rats with osteoarthritis （OA）.Methods Eighteen male SPF Wistar rats,aged 3 months,weighing 200-250 g,were randomly divided into 3 groups （n =6 each） using a random number table：control group （group C）,group OA,and O3 group （group O）.OA was induced by injection of monosodium iodoacetate 3 mg （50 μl） into the right knee joint cavity.On 7th day after the model was established successfully,25 μg/ml O3 1 ml were injected into the knee joint cavity,once a week for 3 consecutive weeks in group O.Behavioral changes were observed after establishment of the model.At 1 day before establishment of the model,and 1,4,7,14,21 and 28 days after establishment of the model,the mechanical paw withdrawal threshold （MWT） was measured.At 28 days after establishment of the model,the total knee joint was removed and stained with haematoxylin and eosin for examination of the pathological changes of the cartilage （under light microscope） and for determination of the expression of β-catenin and matrix metalloproteinase-13 （MMP-13） in the cartilage （by immunohistochemistry）.Results The signs of OA such as hind-limb motor dysfunction,knee joint swelling,or decreased joint motion,and signs of hyperalgesia such as lickings were observed after establishment of the model in rats.Compared with group C,the MWT was significantly decreased at each time point after establishment of the model,and the ex pression of β-catenin and MMP-13 in the cartilage was significantly up-regulated in the other two groups（P〈0.05）.Compared with group OA,the MWT was significantly increased at 7-28 days after establishment of the model,and the expression of β-catenin and MMP-13 in the cartilage was significantly down-regulated in group O （P〈0.05）.The pathological changes of the cartilage were significantly reduced in group O as compared with group OA.Conclusion The mechanism by which O3 mitigates