中文摘要：

基于电化学聚合在金电极表面固定兔抗人免疫球蛋白G抗体与人免疫球蛋白G及标记有Ru（bpy）3^2＋的羊抗人免疫球蛋白G抗体之间发生特异性免疫反应，形成三明治结构，成功建立了用于测定人血清中免疫球蛋白G的电化学发光（ECL）免疫技术。利用此方法测定人免疫球蛋白G含量，浓度在50μg/L-2mg／L范围内与电化学发光强度呈良好的线性关系，线性回归方程为Y（a．u．）=48．41＋0．09x（μg／L）（n=7）；检出限为20μg/L（30σ）。测得正常人血清中免疫球蛋白G平均含量为11．2g／L，结果令人满意。

英文摘要：

Rabbit-anti-human IgG was immobilized on gold electrode by electrochemical copolymerization with pyrrole. An efficient immunoassay strategy for the determination of human IgG in human serum was developed based on the specific immunological reaction between the rabbit-anti-human IgG, human IgG and Ru （bpy）3^2＋-labled goat-anti-human IgG, which formed sandwich-typed immunocomplex. Then the target IgG was determined by electrogenerated chemiluminescence （ECL）. The parameters of polymerization time, incubation time and the pH of detection solution were optimized. The protocol has been characterized with impendence, cyclic vohammogram and ECL. A good linear relationship between ECL intensity and human IgG in the concentration of 50 μg/L - 2 mg/L was obtained, and the detection limit was 20 μg/L.