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中文摘要：
目的建立两基原（决明、小决明）中药决明子的UPLC指纹图谱,为决明子药材质量控制和评价提供依据。方法采用Agilent ZORBAX EP C18（100 mm×3.0 mm,1.8μm）色谱柱;以乙腈-0.01%甲酸水溶液为流动相,梯度洗脱,体积流量0.4 m L/min,检测波长285 nm,柱温30℃,进样量为1μL。结果首次建立了决明子UPLC指纹图谱共有模式,运用相似度评价软件标定了20个共有峰,结合保留时间和紫外光谱分析,指认了7个峰;20批决明子药材相似度均大于0.9;聚类分析结果为当欧式距离平方和为5时,20批决明子样品可分为4类;主成分分析降维得到3个主成分,根据主成分得分将样品进行划分归类,与聚类分析结果一致;通过拟合归纳第一主成分的载荷因子模型,筛选出红链霉素-6-O-β-龙胆二糖苷等具有评价决明子质量优劣的特征峰。结论决明与小决明共有化学成分存在共性差异,该方法可以用于决明子药材质量控制和评价。
英文摘要：
Objective To establish a UPLC fingerprint of Cassiae Semen from two species which is expected to provide standard for the quality control and evaluation. Methods Chromatography conditions were Agilent ZORBAX EP C18（100 mm × 3.0 mm, 1.8 μm） column with gradient mobile phase of acetonitrile-0.01% formic acid solution. UV detection wavelength was 285 nm, the flow rate was 0.4 m L/min, the column temperature was 30 and the sample quantity was 1 ℃ μL. Results The common mode of UPLC fingerprint of Cassiae Semen was established firstly. There were 20 common peaks had been calibrated by similarity evaluation. Seven peaks had been identified by comparing retention time with UV spectrum analysis. The similarity evaluation of 20 batches samples of Cassiae Semen was more than 0.9. Twenty batches of Cassiae Semen could be divided into four grades when the sum of squared Euclidean distance is 5 become the result of Cluster Analysis. Principal component analysis got three principal components through dimension reduction. The results of principal component scores agree with the clustering analysis. By fitting the load factor model of the first principal component, characteristic peak such as rubrofusarin-6-O-β-gentiobioside were filtered to appraise Cassiae Semen＇s quality. Conclusion The contents of same chemical compositions between Cassia obtusifolia and C. tora are different. UPLC fingerprint can be used in Cassiae Semen＇s quality control and evaluation.
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