中文摘要：

目的：采用逐步代入勾兑法控制中药饮片的质量稳定性。方法：选取黄芩为研究对象,以黄芩苷、黄芩素和汉黄芩素为指标成分,采用逐步代入法勾兑,对比勾兑前后各批饮片中指标成分含量与目标饮片的相对标准偏差和指纹图谱相似度差异。结果：勾兑饮片中各指标成分含量与目标饮片的RSD〈5%,勾兑饮片与目标饮片HPLC指纹图谱相似度0.986。结论：不同批次饮片通过逐步代入法勾兑可使指标成分含量的RSD降低和指纹图谱相似度趋于一致,显著提高药材的质量均一性。该方法准确度高、操作简便,不使用复杂数学软件,可作为中药饮片勾兑的新方法。

英文摘要：

Objective： To control quality stability of Chinese materia medica pieces by stepwise blending method.Method： Scutellariae Radix was chosen as research object,with the contents of baicalin,baicalein and wogonin as indexes,five batches of Scutellariae Radix were blended to get one target cruse drug using stepwise blending method,fingerprint similarity was used to verity quality consistency of Scutellariae Radix.Result： RSD of the contents of index components between blending pieces and objective piece was less than 5%,fingerprint similarity between blending pieces and objective piece was 0.986.Conclusion： After different batches of pieces were handled by stepwise blending method,RSD of index components content reduced and fingerprint similarity tended to be consistent,then quality uniformity of Chinese materia medica increased significantly.Stepwise blending method was accurate and simple without complexity mathematical software,which could be a new method for blending of Chinese materia medica pieces.